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Subcellular Fractionation: A Practical Approach

Handbook / Manual

Series: Practical Approach Series Volume: 173

Edited By: JM Graham and D Rickwood

339 pages, Illus, figs, tabs

IRL Press

Paperback | Dec 1997 | #71836 | ISBN: 0199634947
Availability: Usually dispatched within 2-3 weeks Details
NHBS Price: £69.99 $89/€78 approx
Hardback | Dec 1997 | #83992 | ISBN: 0199634955
Out of Print Details

About this book

Many investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component (organelle). This book covers many necessary aspects, from breaking up the cells (homogenization), via a variety of separation techniques (the isolation and fractionation chapters), to characterization of the separated organelles.


Contents

1. Homogenization of tissues and cells; 2. Isolation of subcellular fragments; 3. Isolation and characterization of nuclei and nuclear subfractions; 4. Subcellular fractionation of mitochondria; 5. Isolation and characterization of peroxisomes; 6. Lysosomes and endocytosis; 7. The membranes of the secretory and exocytic pathways; 8. Isolation and purification of functionally intact mitochondria and chloroplasts from plant cells; 9. Ribosomes and polysomes; 10. Electron microscopy of organelles

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