To see accurate pricing, please choose your delivery country.
 
 
United States
£ GBP
All Shops
We're still open for business - read our Brexit and Covid-19 statements

British Wildlife

8 issues per year 84 pages per issue Subscription only

British Wildlife is the leading natural history magazine in the UK, providing essential reading for both enthusiast and professional naturalists and wildlife conservationists. Published eight times a year, British Wildlife bridges the gap between popular writing and scientific literature through a combination of long-form articles, regular columns and reports, book reviews and letters.

Subscriptions from £40 per year

Conservation Land Management

4 issues per year 44 pages per issue Subscription only

Conservation Land Management (CLM) is a quarterly magazine that is widely regarded as essential reading for all who are involved in land management for nature conservation, across the British Isles. CLM includes long-form articles, events listings, publication reviews, new product information and updates, reports of conferences and letters.

Subscriptions from £18 per year
Academic & Professional Books  Organismal to Molecular Biology  Biochemistry & Molecular Biology

Cardiac Gene Expression Methods and Protocols

Handbook / Manual
Series: Methods in Molecular Biology Volume: 366
Edited By: Jun Zhang and Gregg Rokosh
Publisher: Humana Press
Cardiac Gene Expression
Click to have a closer look
  • Cardiac Gene Expression ISBN: 9781588293527 Hardback Dec 2006 Usually dispatched within 1-2 weeks
    £127.50
    #163614
Price: £127.50
About this book Contents Customer reviews Related titles

About this book

Cardiac Gene Expression: Methods and Protocols presents both cutting-edge and established methods for studying cardiac gene expression. The protocols provide a template for solid research, and cover the process through screening, analysis, characterization, and functional confirmation of novel genes or known genes with a new function. Section I, Cardiac Gene Expression Profiling: The Global Perspective, discusses several different approaches to examining, identifying, and analyzing changes in transcriptome gene expression. Section II, Cardiac Gene Regulation: Gene-Specific mRNA Measurement in the Myocardium, outlines more sensitive and gene-targeted expression methods. Section III, Cardiac Gene Regulation: Promoter Characterization in the Myocardium, provides protocols for the study of underlying gene regulation mechanisms by focusing on the interaction of transcription factors with their cognate cis binding elements. Section IV, In Silico Assessment of Regulatory cis-Elements and Gene Regulation, and Section V, Cardiac Single Network Polymorphisms, emphasize new analytical approaches for deciphering the functional elements buried in the 3 billion nucleotides of the human genome and other model genomes. The concluding section, Gene Overexpression and Targeting in the Myocardium, highlights methods that facilitate overexpression or cardiac-specific targeted gene deletion.

Contents

Table of Contents; Preface; Contributors; PART I. Cardiac gene expression profiling; The global perspective; 1. Microarray analysis of gene expression in murine cardiac graft infiltrating cells; Yurong Liang, Xin Lu, David L. Perkins; 2. Expression Profiling using Affymetrix GeneChip[registered] Probe Arrays; Martina Schinke-Braun, Jennifer A. Couget; 3. Serial analysis of gene expression (SAGE): A useful tool to analyze the cardiac transcriptome; Kirill V. Tarasov, Sheryl A. Brugh, Yelena S. Tarasova, Kenneth R. Boheler; 4. Functional genomics by cDNA subtractive hybridization; Christophe Depre; 5. Statistical methods in cardiac gene expression profiling: from image to function; Sek Won Kong; PART II. Cardiac gene regulation- gene-specific mRNA measurement in the myocardium; 6. Measurement of cardiac gene expression by reverse transcription polymerase chain reaction (RT-PCR); Nicola King; 7. Quantitative (real-time) RT-PCR in cardiovascular research; Kevin John Ashton, John Patrick Headrick; 8. RNase protection assay for quantifying gene expression levels; Yongxia Qu, Mohamed Boutjdir; 9. In situ hybridization, a technique to study localization of cardiac gene expression; Thierry P. Calmels, David Mazurais; PART III. Cardiac gene regulation- promoter characterization in the myocardium; 10. Characterization of cis-regulatory elements and transcription factor binding: Gel mobility shift assay Jim Jung-Ching Lin, Shaun E. Grosskurth, Shannon M. Jaacks, Elisabeth A. Gustafson-Wagner, Qin Wang. 11. Mapping transcriptional start sites and in silico DNA footprinting.; Martin E Cullen, Paul JR Barton; 12. Characterization of cardiac gene promoter activity: reporter constructs and heterologous promoter studies; Hsiao-Huei Chen, Alexandre F. R. Stewart; PART IV. In Silico assessment of regulatory cis-elements and gene regulation; 13. Comparative genomics: A Tool to functionally annotate human DNA; Jan-Fang Cheng, James R. Priest, Len A. Pennacchio; 14. Developing computational resources in cardiac gene expression; Michael B. Bober, Raimond Winslow; PART V. Cardiac single nucleotide polymorphisms; 15. in silico analysis of SNPs and other high throughput data; Neema Jamshidi, Thuy D. Vo, Bernhard O. Palsson; 16. Discovery and identification of sequence polymorphisms and mutations with MALDI-TOF Ms; Dirk van den Boom, Mathias Ehrich; PART VI. Gene overexpression and targeting in the myocardium; 17. Conditional targeting: Inducible deletion by Cre recombinase; Kelly R O'Neal, Ramtin Agah; 18. Cardiomyocyte preparation, culture, and gene transfer; Alexander H. Maass, Massimo Buvoli; 19. Adeno-associated viral vector delivered hypoxia-inducible gene expression in ischemic hearts; Hua Su, Yuet Wai Kan.

Customer Reviews

Handbook / Manual
Series: Methods in Molecular Biology Volume: 366
Edited By: Jun Zhang and Gregg Rokosh
Publisher: Humana Press
Media reviews

...a timely, detailed and well edited methodss text which would be a valuable addition to any cardiovascular research laboratory. -Molecular Biotechnology

Current promotions
British WildlifeField Studies CouncilSpecial Offer: Ecology and Natural History bookOrder your free copy of our 2021 equipment catalogues