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RNAi and Plant Gene Function Analysis: Methods and Protocols

Handbook / Manual

Series: Methods in Molecular Biology Volume: 744

Edited By: Hiroaki Kodama and Atsushi Komamine

224 pages, 43 illus

Humana Press

Hardback | May 2011 | #191739 | ISBN-13: 9781617791222
Availability: Usually dispatched within 1-2 weeks Details
NHBS Price: £109.99 $144/€125 approx

About this book

The use of RNAi technology is essential for most plant science researchers. As DNA sequence information increases, so the need for functional annotation of target genes also increases. This volume seeks to serve as an ideal guide to scientists of all backgrounds looking to further their knowledge of this fundamental science.


1. Overview of Plant RNAi Yuichiro Watanabe 2. Caveat of RNAi in Plants: the Off-Target Effect Muthappa Senthil-Kumar and Kirankumar S. Mysore 3. Plant Gateway Vectors for RNAi as a Tool for Functional Genomic Studies Toshiya Muranaka 4. Heat-Inducible RNAi for Gene Functional Analysis in Plants Frederic Masclaux and Jean-Philippe Galaud 5. Gene Function Analysis by Artificial MicroRNAs in Physcomitrella patens Basel Khraiwesh, Isam Fattash, M. Asif Arif, and Wolfgang Frank 6. Virus-Induced Gene Silencing in Ornamental Plants Cai-Zhong Jiang, Jen-Chih Chen, and Michael Reid 7. Local RNA Silencing Mediated by Agroinfiltration Jutta Maria Helm, Elena Dadami, and Kriton Kalantidis 8. Direct Transfer of Synthetic Double-Stranded RNA into Protoplasts of Arabidopsis thaliana Ha-il Jung, Zhiyang Zhai and Olena K. Vatamaniuk 9. Detection of Long and Short Double-Stranded RNAs Toshiyuki Fukuhara, Syunichi Urayama, Ryo Okada, Eri Kiyota, and Hiromitsu Moriyama 10. Quantitative Stem-Loop RT-PCR for Detection of MicroRNAs Erika Varkonyi-Gasic and Roger P. Hellens 11. Large Scale Sequencing of Plant Small RNAs William P. Donovan, Yuanji Zhang, and Miya D. Howell 12. Computational Prediction of Plant miRNA Targets Ying-Hsuan Sun, Shanfa Lu, Rui Shi, and Vincent L. Chiang 13. Bisulfite Sequencing for Cytosine-Methylation Analysis in Plants Nazmul Haque and Masamichi Nishiguchi 14. Using Nuclear Run-On Transcription Assays in RNAi Studies Basel Khraiwesh 15. Proteomic Analysis of RNA-Interference Induced Knockdown Plant Sang Yeol Lee and Kyun Oh Lee 16. Comparative Analysis of Phosphoprotein Expression using 2D-DIGE Tomoya Asano and Takumi Nishiuchi

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