Plant Genome Editing with CRISPR Systems Methods and Protocols

About this book

This volume provides readers with wide-ranging coverage of CRISPR systems and their applications in various plant species. The chapters in this book discuss topics such as plant DNA repair and genome editing; analysis of CRISPR-induced mutations; multiplexed CRISPR/Cas9 systems; CRISPR-Cas12a (Cpf1) editing systems; and non-agrobacterium based CRISPR delivery systems. Written in the standard Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Plant Genome Editing with CRISPR Systems: Methods and Protocols is a valuable resource for any researcher interested in learning about and using CRISPR systems in plants.

Contents

Preface
Table of Contents
Contributing Authors

Part I Review on Plant DNA Repair and Genome Editing
1. Plant DNA Repair Pathways and their Applications in Genome Editing / Qiudeng Que, Zhongying Chen, Tim Kelliher, David Skibbe, Shujie Dong, and Mary-Dell Chilton

Part II CRISPR Design and Mutation Analysis
2. Rapid Screening of CRISPR/Cas9-Induced Mutants using the ACT-PCR Method / Chun Wang and Kejian Wang
3. Decoding Sanger-Sequencing Chromatograms from CRISPR-Induced Mutations / Xianrong Xie, Xingliang Ma, and Yao-Guang Liu

Part III Multiplexed CRISPR-Cas9 Systems
4. Creating Large Chromosomal Deletions in Rice using CRISPR/Cas9 / Riqing Li, Si Nian Char, and Bing Yang
5. A Multiplexed CRISPR-Cas9 Editing System Based on the Endogenous tRNA Processing / Kabin Xie and Yinong Yang
6. A Single Transcript CRISPR-Cas9 System for Multiplex Genome Editing in PlantsXu Tang, Zhaohui Zhong, Qiurong Ren, Binglin Liu, and Yong Zhang
7. CRISPR-Act2.0: An Improved Multiplexed System for Plant Transcriptional Activation / Aimee Malzahn, Yong Zhang, and Yiping Qi

Part IV CRISPR-Cas9 Editing in Monocots
8. Generating Photoperiod-Sensitive Genic Male Sterile Rice Lines with CRISPR-Cas9 / Weihang Gu, Dabing Zhang, Yiping Qi, and Zheng Yuan
9. Knocking Out MicroRNA Genes in Rice with CRISPR/Cas9 / Jianping Zhou, Zhaohui Zhong, Hongqiao Chen, Mengqiao Yang, Qian Li, Xuelian Zheng, Yiping Qi, and Yong Zhang
10. An Agrobacterium-Mediated CRISPR-Cas9 Platform for Genome Editing in Maize / Keunsub Lee, Huilan Zhu, Bing Yang, and Kan Wang

Part V CRISPR-Cas9 Editing in Dicots
11. Fluorescence Marker-Assisted Isolation of Cas9-Free and CRISPR-Edited Arabidopsis Plants / Hanchuanzhi Yu and Yunde Zhao
12. Creating Targeted Gene Knock-Outs in Brassica oleracea using CRISPR/Cas9 / Tom Lawrenson, Penny Hundleby, and Wendy Harwood
13. Application of CRISPR/Cas9-Mediated Gene Editing in Tomato / Nathan T. Reem and Joyce Van Eck
14. Genome Editing in Potato with CRISPR/Cas9 / Satya Swathi Nadakuduti, Colby G. Starker, Daniel F. Voytas, C. Robin Buell, and David S. Douches
15. Visual Assay for Gene Editing using a CRISPR/Cas9 System in Carrot Cells / Magdalena Klimek-Chodacka, Tomasz Oleszkiewicz, and Rafal Baranski
16. Genome Editing in Soybean with CRISPR/Cas9 / Junqi Liu, Samatha Gunapati, Nicole T. Mihelich, Adrian O. Stec, Jean-Michel Michno, and Robert M. Stupar
17. Genome Editing in Citrus Tree with CRISPR/Cas9 / Hongge Jia, Xiuping Zou, Vladimir Orbovic, and Nian Wang

Part VI CRISPR-Cas12a Editing Systems
18. Plant Gene Knockout and Knockdown by CRISPR-Cpf1 (Cas12a) Systems / Yingxiao Zhang, Yong Zhang, and Yiping Qi
19. Editing a Stomatal Developmental Gene in Rice with CRISPR/Cpf1 / Abhishek Anand, Xiaojia Yin, Paul Quick, and Anindya Bandyopadhyay
20. Targeted Mutagenesis using FnCpf1 in Tobacco / Akira Endo and Seiichi Toki

Part VII Precise Gene Editing
21. Gene Replacement by Intron Targeting with CRISPR-Cas9 / Jun Li, Xiangbing Meng, Jiayang Li, and Caixia Gao
22. Targeted Base Editing with CRISPR-Deaminase in Tomato / Zenpei Shimatani, Tohru Ariizumi, Ushio Fujikura, Akihiko Kondo, Hiroshi Ezura, and Keiji Nishida

Part VIII Non-Agrobacterium Based CRISPR Delivery Systems
23. Virus-Mediated Genome Editing in Plants using the CRISPR/Cas9 System / Ahmed Mahas, Zahir Ali, Manal Tashkandi, and Magdy M. Mahfouz
24. Biolistic Delivery of CRISPR/Cas9 with Ribonucleoprotein Complex in Wheat / Zhen Liang, Kunling Chen, and Caixia Gao
25. DNA-Free Genome Editing via Ribonucleoprotein (RNP) Delivery of CRISPR/Cas in Lettuce / Jongjin Park, Aiden Y. Park, Slki Park, Jiyoung Yoon, Sunmee Choi, and Sunghwa Choe
26. An Automated Protoplast Transformation System / Scott C. Lenaghan and C. Neal Stewart, Jr

Customer Reviews