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A gold standard collection of Agrobacterium-mediated transformation techniques for state-of-the-art plant genetic engineering, functional genomic analysis, and crop improvement. Volume 1 details the most updated techniques available for twenty-six plant species drawn from cereal crops, industrial plants, legume plants, and vegetable plants, and presents various methods for introducing DNA into three major model plant species, Arabidopsis thaliana, Medicago truncatula, and Nicotiana. The authors also outline the basic methods in Agrobacterium manipulation and strategies for vector construction. Volume 2 contains another thirty-three proven techniques for root plants, turf grasses, woody species, tropic plants, nuts and fruits, ornamental plants, and medicinal plants. Additional chapters provide methods for introducing DNA into non-plant species, such as bacteria, fungi, algae, and mammalian cells. The protocols follow the successful Methods in Molecular BiologyT series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Part I. Agrobacterium Handling Culture and Maintenance of Agrobacterium Strains Arlene A. Wise, Zhenying Liu, and Andrew N. Binns Binary Vectors and Super-binary Vectors Toshihiko Komari, Yoshimitsu Takakura, Jun Ueki, Norio Kato, Yuji Ishida, and Yukoh Hiei Three Methods for the Introduction of Foreign DNA into Agrobacterium Arlene A. Wise, Zhenying Liu, and Andrew N. Binns Integration of Genes into the Chromosome of Agrobacterium tumefaciens C58 Lan-Ying Lee Nucleic Acid Extraction from Agrobacterium Strains Arlene A. Wise, Zhenying Liu, and Andrew N. Binns Agrobacterium Virulence Gene Induction Stanton B. Gelvin Part II. Model Plants Arabidopsis thaliana Floral Dip Transformation Method Andrew Bent Agrobacterium Transformation of Arabidopsis thaliana Roots: A Quantitative Assay Stanton B. Gelvin Medicago truncatula Transformation Using Leaf Explants Viviane Cosson, Patricia Durand, Isabelle d'Erfurth, Adam Kondorosi, and Pascal Ratet Medicago truncatula Transformation Using Cotyledon Explants Elane Wright, Richard A. Dixon, and Zeng-Yu Wang Medicago truncatula Transformation Using Root Explants Cynthia Crane, Richard A. Dixon, and Zeng-Yu Wang Nicotiana (Nicotiana tobaccum, Nicotiana benthamiana) Tom Clemente Generation of Composite Plants Using Agrobacterium rhizogenes Christopher G. Taylor, Beth Fuchs, Ray Collier, and W. Kevin Lutke Part III. Cereal Crops Barley (Hordeum vulgare L.) John Jacobsen, Ingrid Venables, Ming-Bo Wang, Peter Matthews, Michael Ayliffe, and Frank Gubler Maize (Zea mays L.) Bronwyn R. Frame, Tina Paque, and Kan Wang Indica Rice (Oryza sativa, BR29 and IR64) Karabi Datta and Swapan Kumar Datta Japonica Rice Varieties (Oryza sativa, Nipponbare, and Others) Philippe Herve and Toshiaki Kayano Rye (Secale cereale L.) Fredy Altpeter Sorghum (Sorghum bicolor L.) Zuo-yu Zhao Wheat (Triticum aestivum L.) Yuechun Wan and Jeanne Layton Part IV. Industrial Plants Canola (Brassica napus L.) Vinitha Cardoza and C. Neal Stewart, Jr. Cotton (Gossypium hirsutum L.) Keerti S. Rathore, Ganesan Sunilkumar, and LeAnne M. Campbell Indian Mustard [Brassica juncea (L.) Czern.] Ksenija Gasic and Schuyler S. Korban Sunflower (Helianthus annuus L.) Dalia M. Lewi, H. Esteban Hopp, and Alejandro S. Escandon Part V. Legume Plants Alfalfa (Medicago sativa L.) Deborah A. Samac and Sandra Austin-Phillips Chickpea (Cicer arietinum L.) Kiran Kumar Sharma, Pooja Bhatnagar-Mathur, and Boddu Jayanand Clovers (Trifolium spp.) Aidyn Mouradov, Stephen Panter, Marcel Labandera, Emma Ludlow, Michael Emmerling, and German Spangenberg Peas (Pisum sativum L.) Jan Grant and Pauline Cooper Peanut (Arachis hypogaea L.) Kiran Kumar Sharma and Pooja Bhatnagar-Mathur Pigeonpea (Cajanus cajan L. Millsp.) Kiran Kumar Sharma, Gopinath Sreelatha, and Sunitha Dayal Red Clover (Trifolium pratense) Michael L. Sullivan and Kenneth H. Quesenberry Soybean (Glycine max) Transformation Using Mature Cotyledonary Node Explants Paula M. Olhoft, Christopher M. Donovan, and David A. Somers Soybean (Glycine max) Transformation Using Immature Cotyledon Explants Tae-Seok Ko, Schuyler S. Korban, and David A. Somers Tepary Bean (Phaseolus acutifolius) Mukund Zambre, Marc Van Montagu, Geert Angenon, and Nancy Terryn Part VI. Vegetable Plants Brassica oleracea Penny A. C. Sparrow, Philip J. Dale, and Judith A. Irwin Cucumber (Cucumis sativus L.) Wojciech Burza, Sabina Zuzga, Zhimin Yin, and Stefan Malepszy Eggplant (Solanum melongena L.) Joyce Van Eck and Ada Snyder Lettuce (Lactuca sativa L.) Ian S. Curtis Tomato (Lycopersicum esculentum) Joyce Van Eck, Dwayne D. Kirk, and Amanda M. Walmsley Index
From the reviews of the second edition: "This book is intended as a laboratory manual for researchers using Agrobacterium tumefaciens for plant transformation. It covers the basics of culturing and transforming Agrobacterium ! . You don't expect a lab manual to be ideal ! reading, but I found this to be surprisingly readable. ! The book would be especially useful for someone just starting plant transformation work or changing to new plant species and ! it would also be useful reading for Masters students." (Heather Macdonald, Microbiology Today, November, 2006) "Methods in Molecular Biology opens ! a field concerning Agrobacterium-mediated transformation of plants. ! Each chapter is written by the leader in the field ! and offers a detailed manual of the transformation protocol. ! All users of this book will certainly appreciate the notes sections that bring additional information on potential difficulties in the protocols and alternative materials or methods. The book is the fountainhead of recent practical knowledge concerning Agrobacterium-mediated transformation and ! it will become the core manual ! ." (J. BRlZA, Biologia Plantarum, Vol. 51 (2), 2007) "The book's chapters are organized in a highly detailed fashion, providing readers not only with step-by-step protocols ! but also with a comprehensive list of equipment and materials. ! With such a rich source of information, organized to be easy to read and very simple to implement, the book should be considered a must for every plant biology laboratory. ! It will also be an excellent tool for training students, doctorates, technicians, and other laboratory personnel in the art of plant genetic transformation." (Tzvi Tzfira, Quarterly Review of Biology, Vol. 82, March, 2007)