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A versatile collection of readily reproducible cell-cell interaction assays for uncovering cellular interactions at the molecular level, both in vitro and in vivo. The protocols cover a diverse set of cell-cell interaction models in both normal and pathological states, are readily adaptable to nearly any cell type and organ system, and include primary data and outcome analysis. In addition, the protocols follow the successful Methods in Molecular BiologyT series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Blood-Brain Barrier and Cell-Cell Interactions: Methods for Establishing In Vitro Models of the Blood-Brain Barrier and Transport Measurements Michael Aschner, Vanessa A. Fitsanakis, Ana Paula Marreilha dos Santos, Luisa Olivi, and Joseph P. Bressler Interactions of Intestinal Epithelial Cells With Bacteria and Immune Cells: Methods to Characterize Microflora and Functional Consequences Geraldine Canny, Alexander Swidsinski, and Beth A. McCormick The Role of Junctional Adhesion Molecules in Interactions Between Vascular Cells Triantafyllos Chavakis and Valeria Orlova Cell-Cell Interactions on Solid Matrices Nancy A. Louis, Dionne Daniels, and Sean P. Colgan Cell-Cell Interactions in the Kidney: Inducible Expression of Mutant G Protein a-Subunits in Madin-Darby Canine Kidney Cells for Studies of Epithelial Cell Junction Structure and Function Ernesto Sabath and Bradley M. Denker Nucleotide Metabolism and Cell-Cell Interactions Holger K. Eltzschig, Thomas Weissmuller, Alice Mager, and Tobias Eckle Analysis of Mammalian Sperm-Egg Membrane Interactions During In Vitro Fertilization Genevieve B. Wortzman, Allison J. Gardner, and Janice P. Evans Collagen Gel Contraction Assay Peter Ngo, Punitha Ramalingam, Jonathan A. Phillips, and Glenn T. Furuta Methods to Assess Tissue Permeability Juan C. Ibla and Joseph Khoury Bacterial-Bacterial Cell Interactions in Biofilms: Detection of Polysaccharide Intercellular Adhesins by Blotting and Confocal Microscopy Kimberly K. Jefferson and Nuno Cerca A Biochemical Method for Tracking Cholera Toxin Transport From Plasma Membrane to Golgi and Endoplasmic Reticulum Heidi E. De Luca and Wayne I. Lencer Isolation and Culture of Murine Heart and Lung Endothelial Cells for In Vitro Model Systems Yaw-Chyn Lim and Francis W. Luscinskas Expression Cloning of Signaling Proteins Regulated by Cell Adhesion Michelle L. Matter and Joe W. Ramos Assays for the Calcium Sensitivity of Desmosomes Anita J. Merritt, Anthea Scothern, and Tanusree Bhattacharyya Tight Junctions and Cell-Cell Interactions Markus Utech, Matthias Bruwer, and Asma Nusrat Simultaneous Immunofluorescence Detection of Cadherin and b-Catenin in Mouse Tissue Sections Nobuko Obara In Vitro Neutrophil Transepithelial Migration Winston Y. Lee, Alex C. Chin, Susan Voss, and Charles A. Parkos The Blot Rolling Assay: A Method for Identifying Adhesion Molecules Mediating Binding Under Shear Conditions Robert Sackstein and Robert Fuhlbrigge Cell-Cell Interaction in the Transcellular Biosynthesis of Novel w-3-Derived Lipid Mediators Nan Chiang and Charles N. Serhan Isolation and Analysis of Lipid Rafts in Cell-Cell Interactions Aimee Landry and Ramnik Xavier Index