Cell Senescence Methods and Protocols

About this book

Cell senescence is the process whereby cells permanently lose the possibility to proliferate without undergoing cell death, and occurs in a plethora of distinct model organisms. In Cell Senescence: Methods and Protocols, expert researchers in the field detail the methods that are now commonly used to study cell senescence, in model organisms encompassing bacteria, fungi, worms, flies, zebrafish, and mammalian cells. These techniques cover the study of all the morphological, biochemical and functional manifestations of senescence at the cellular level and include protocols for population analyses and high-throughput approaches in suitable model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Contents

1. Cell Senescence in Health and Disease Wataru Kudo, Jaewon Chang, Gemma Casadesus, Hyoung-gon Lee
2. Cell Senescence as Both a Dynamic and a Static Phenotype Andrew RJ Young, Masako Narita, and Masahi Narita
3. Senescence Regulation by mTOR Vjekoslav Dulic
4. Senescence Regulation by the p53 Protein Family Yingjuan Qian and Xinbin Chen
5. Markers of Cellular Senescence Amancio Carnero
6. Biomarkers of Cell Senescence Assessed by Imaging Cytometry Hong Zhao and Zbigniew Darzynkiewicz
7. Cytofluorometic Assessment of Cell Cycle Progression Ilio Vitale, Mohamed Jemaa, Lorenzo Galluzzi, Didier Metivier, Maria Castedo and Guido Kroemer
8. Quantification of Cell Cycle-arresting Proteins Oliver Kepp, Isabelle Martins, Laurie Menger, Mickael Michaud, Sandy Adjemian, Abdul Qader Sukkurwala, Lorenzo Galluzzi, and Guido Kroemer
9. Colorimetric Detection of Senescence-associated Beta Galactosidase (SA-Betagal)
10. Chemiluminescent Detection of Senescence-Associated Beta Galactosidase (SA-Betagal)
11. Detection of the Senescence-associated Secretory Phenotype(SASP) Francis Rodier
12. Unbiased Characterization of the Senescence-associated Secretome using SILAC-Based Quantitative Proteomics Juan Carlos Acosta, Ambrosius P. Snijders, and Jesus Gil
13. Detection of Senescence-associated Heterochromatin Foci (SAHF) Katherine M. Aird and Rugang Zhang
14. Monitoring DNA Damage During Cell Senescence Glyn Nelson and Thomas von Zglinicki
15. Assessment and Quantification of Telomerase Enzyme Activity Michelle F. Maritz, Laura A. Richards, Karen L. MacKenzie
16. Methods for the Assessment of Telomere Status Asako J. Nakamura
17. Detection of Nuclear Envelope Alterations in Senescence Clea Barcena, Fernando G. Osorio and Jose M.P. Freije
18. Measuring Reactive Oxygen Species in Senescent Cels Joao F. Passos, Satomi Miwa, and Thomas von Zglinicki
19. Quantification of Protein Carbonylation Nancy D. Wehr and Rodney L. Levine
20. Assays for the Measurement of Lipid Peroxidation Ana Cipak Gasparovic, Morana Jaganjac, Branka Mihaljevic, Suzana Borovic Sunjic, and Neven Zarkovic
21. Raman Spectroscopy for the Detection of AGEs/ALEs J. Renwich Beattie, John J. McGarvey, Alan W. Stitt
22. Monitoring Oncogenic B-RAF-induced Senscence in Melanocytes Sieu L. Tran and Helen Rizos
23. Methods to Investigate the Role of SIRT1 in Endothelial Senescence Bo Bai and Yu Wang
24. Monitoring Nutrient Signaling Through the Longevity Protein p66SHC1 Sofia Chiatamone Ranieri and Giovambattista Pani
25. Profiling the Metabolic Signature of Senescence Florian M. Geier, Silke Fuchs, Gabriel Valbuena, Armand M. Leroi, and Jacob G. Bundy
26. Genome-wide RNAi Screening to Identify Regulators of Oncogene-induced Cellular Senescence Narendra Wajapeyee, Sara Deibler, and Michael R. Gree
27. An Integrated Approach for Monitoring Cell Senescence Tatiana V. Pospelova, Zhanna V. Chitikova, Valery A. Pospelov
28. Robust Multiparametric Assessment of Cellular Senescence Clara Correia-Melo, Diana Jurk and Joao F. Passos
29. Assessing Chronological Aging in Bacteria Stavros Gonidakis and Valter D. Longo
30. Assessing Organismal Aging in the Filamentous Fungus Podospora anserina Heinz D. Osiewacz, Andrea Hamann, Sandra Zintel
31. Assessing Chronological Aging in Saccharomyces cerevisiae Jia Hu, Min Wei, Mario G. Mirisola, and Valter D. Longo
32. Assessing Aging and Senescent Decline in Caenorhabditis elegans: Cohort Survival Analysis Eirini Lionaki and Nektarios Tavernarakis
33. High Throughput and Longitudinal Analysis of Aging and Senescent Decline in Caenorhabditis elegans Eirini Lionaki and Nektarios Tavernarakis
34. Assessing Senescence in Drosophila using Video Tracking Reza Ardekani, Simon Tavare and John Tower
35. Assessing Vascular Senescence in Zebrafish Sandra Donnini, Antonio Giachetti, and Marian Ziche

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