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In this updated second edition, leading researchers apply molecular diagnostics to the many recent advances that have occurred in polymerase chain reaction( PCR)-based technologies. Highlights include real-time PCR, which allows the technique to be performed in a quantitative manner with improved sensitivity, robustness, and resilience to carryover contamination, mass spectrometric analysis of nucleic acids, and circulating cell-free nucleic acids in plasma. The authors apply these innovations to a broad spectrum of applications, including gene expression, methylation, trace molecule, gene dosage, and single cell analysis.
Introduction to the Polymerase Chain Reaction Y. M. Dennis Lo and K. C. Allen Chan Setting Up a Polymerase Chain Reaction Laboratory Y. M. Dennis Lo and K. C. Allen Chan Real-Time Polymerase Chain Reaction and Melting Curve Analysis Robert J. Pryor and Carl T. Wittwer Qualitative and Quantitative Polymerase Chain Reaction-Based Methods for DNA Methylation Analyses Ivy H. N. Wong In-Cell Polymerase Chain Reaction: Strategy and Diagnostic Applications T. Vauvert Hviid Qualitative and Quantitative DNA and RNA Analysis by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Chunming Ding Analysis of Polymerase Chain Reaction Products by Denaturing High-Performance Liquid Chromatography Ching-Wan Lam Use of Real-Time Polymerase Chain Reaction for the Detection of Fetal Aneuploidies Bernhard Zimmermann, Lisa Levett, Wolfgang Holzgreve, and Sinuhe Hahn Noninvasive Prenatal Diagnosis by Analysis of Fetal DNA in Maternal Plasma Rossa W. K. Chiu and Y. M. Dennis Lo Clinical Applications of Plasma Epstein-Barr Virus DNA Analysis and Protocols for the Quantitative Analysis of the Size of Circulating Epstein-Barr Virus DNA K. C. Allen Chan and Y. M. Dennis Lo Molecular Analysis of Circulating RNA in Plasma Nancy B. Y. Tsui, Enders K. O. Ng, and Y. M. Dennis Lo Molecular Analysis of Mitochondrial DNA Point Mutations by Polymerase Chain Reaction Lee-Jun C. Wong, Bryan R. Cobb, and Tian-Jian Chen Novel Applications of Polymerase Chain Reaction to Urinary Nucleic Acid Analysis Anatoly V. Lichtenstein, Hovsep S. Melkonyan, L. David Tomei, and Samuil R. Umansky Detection and Quantitation of Circulating Plasmodium falciparum DNA by Polymerase Chain Reaction Shira Gal and James S. Wainscoat Molecular Diagnosis of Severe Acute Respiratory Syndrome Enders K. O. Ng and Y. M. Dennis Lo Genomic Sequencing of the Severe Acute Respiratory Syndrome-Coronavirus Stephen S. C. Chim, Rossa W. K. Chiu, and Y. M. Dennis Lo Index
From the reviews: "...will be an important laboratory resource to those using PCR in the diagnosis of human diseases and should be of great interest to both molecular and clinical pathologists." -Archives of Pathology and Laboratory Medicine "Each chapter is clearly written and provides enough background material to explain the significance and application of the procedure. The protocols include sufficient detail to enable the reader to develop the test. This manual will be an important laboratory resource to those using PCR in the diagnosis of human diseases and should be of great interest to both molecular and clinical pathologists."-Archives of Pathology and Laboratory Medicine "...a unique document that will be welcome in the clinical laboratory as well as the research field." -Doody's Health Sciences Book Review Journal "This book is pitched at both pathologists and microbiologists who use PCR in a diagnostic setting. It provides a user-friendly and relatively brief theoretical background to conventional and real-time PCR ! . This book should appeal to professional laboratory scientists and students ! as well as to more experienced individuals who want to use or extend specific applications. This book would be a useful purchase as a reference source and technical manual for diagnostic pathology and microbiology laboratories." (David Hampson, SGM- Society for General Microbiology, 2007)