Over the last two decades there has been a growing interest in cyclic nucleotide research in plants with an emphasis on the elucidation of the roles of cGMP and cAMP. In Cyclic Nucleotide Signaling in Plants: Methods and Protocols, expert researchers in the field detail many approaches to better understand the biological role of this important signaling system.
Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cyclic Nucleotide Signaling in Plants: Methods and Protocols seeks to aid scientist in further understanding signal transduction and the molecular mechanisms underlying cellular signaling.
1: Molecular Methods for the Study of Signal Transduction in Plants Helen R. Irving and Chris Gehring
2: Recombinant Expression and Functional Testing of Candidate Adenylate Cyclase Domains Oziniel Ruzvidzo, Bridget T. Dikobe, David T. Kawadza, Grace H. Mabadahanye, Patience Chatukuta, Lusisizwe Kwezi
3: Quantification of Cyclic Dinucleotides by Reversed-Phase LC-MS/MS Heike Burhenne and Volkhard Kaever
4: Determination of ADP-ribosyl Cyclase Activity, Cyclic ADP-ribose and Nicotinamide Adenine Dinucleotide Phosphate in Tissue Extracts Richard M. Graeff and Hon Cheung Lee
5: In vivo Imaging of cGMP in Plants Jean-Charles Isner and Frans J.M. Maathuis
6: Characterization of Heterologously Expressed Transporter Genes by Patch- and Voltage-Clamp Methods: Application to Cyclic Nucleotide-Dependent Responses Fouad Lemtiri-Chlieh and Rashid Ali
7: Non-invasive Microelectrode Ion Flux Estimation Technique (MIFE) for the Study of the Regulation of Root Membrane Transport by Cyclic Nucleotides Natalia Maria Ordonez, Lana Shabala, Chris Gehring, and Sergey Shabala
8: Calcium Imaging of the Cyclic Nucleotide Response Martin R. McAinsh, Stephen K. Roberts and Lyudmila V. Dubovskaya
9: Identification and Quantification of Signal Molecule-dependent Protein Phosphorylation Arnoud Groen, Ludivine Thomas, Kathryn Lilley and Claudius Marondedze
10: Comparative Gel-based (Phospho)Proteomics in Response to Signaling Molecules Claudius Marondedze, Kathryn Lilley and Ludivine Thomas
11: An Affinity Pull-down Approach to Identify the Plant Cyclic Nucleotide Interactome Lara Donaldson and Stuart Meier
12: Structural and Functional Characterization of Receptor Kinases with Nucleotide Cyclase Activity Victor Muleya, Janet I. Wheeler and Helen R. Irving
13: Computational Identification of Candidate Nucleotide Cyclases in Higher Plants Aloysius Wong and Chris Gehring
14: Identification of Cyclic Nucleotide Gated Channels Using Regular Expressions Alice K. Zelman, Adam Dawe and Gerald A. Berkowitz
15: Inferring biological functions of guanylyl cyclases with computational methods May Alqurashi and Stuart Meier
16: Identification and Characterization of Cyclic Nucleotide Phosphodiesterases Erin B. Purcell and Rita Tamayo
17: Detection of reactive oxygen species down-stream of cyclic nucleotide signals in plants Robin K. Walker and Gerald A. Berkowitz
18: Measurement of Nitric Oxide in Plant Tissue using Difluorofluorescein and Oxyhaemoglobin Ndiko Ludidi
19: Infra-red Gas Analysis (IRGA) Technique for the Study of the Regulation of Photosynthetic Responses Alex Valentine, Oziniel Ruzvidzo, Aleysia Kleinert, Yun Kang and Vagner Bennedito
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