Cytokine Protocols

About this book

Recent studies have discovered new known and characterized cytokines, allowing for advancement in miniaturization of micro-analytical methods as well as the extensive development of bio-informatics and nanotechnology. These advancements have allowed researchers to reduces sample sizes making for more accurate determinations then previously possible.

In Cytokine Protocols, expert researchers in the field detail many of the methods which are now commonly used to study cytokines. These methods and techniques for studying cytokines include historical importance and the importance of researchers using bioassay, quantification, and characterization of cytokine related RNAs, posttranscriptional modifications of RNA, either naturally or artificially, and observations at the protein level.

Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Authoritative and practical, Cytokine Protocols seeks to aid scientists in furthering the crucially important advancement of cytokine research.

Contents

1 A Tale of Two Interferon Bioassays: How Frustration with Discrepant Results from Slightly Dissimilar Methods can Engender Discovery; Alfons Billiau
2 The use of Real-Time Quantitative PCR (RT-qPCR) for the Analysis of Cytokine mRNA Levels; Maria Forlenza, Thomas Kaiser, Huub F.J. Savelkoul, and Geert F. Wiegertjes
3 Interleukin-27 Induces Interferon-Inducible Genes: Analysis of Gene Expression Profiles using Affymetrix Microarray and DAVID; Tomozumi Imamichi, Jun Yang, Da Wei Huang, Brad Sherman, and Richard A. Lempicki
4 Quantitative Analysis of miRNA Expression in Epithelial Cells and Tissues; Markus Bitzer, Wenjun Ju, Xiahong Jing and Jiri Zavadil
5 Evaluating Posttranscriptional Regulation of Cytokine Genes; Bernd Rattenbacher and Paul R. Bohjanen
6 Cloning of Cytokine 3' Untranslated Regions and Post-Transcriptional Assessment using Cell-Based GFP Assay; Latifa Al-Haj and Khalid S.A. Khabar
7 Integrin Targeted Stabilized Nanoparticles for an Efficient Delivery of siRNAs in vitro and in vivo; Charudharshini Srinivasan, Dan Peer, and Motomu Shimaoka
8 Hammerhead Ribozyme Mediated Knockdown of mRNA for Fibrotic Growth Factors: Transforming Growth Factor-Beta 1 and Connective Tissue Growth Factor; Paulette M. Robinson, Timothy D. Blalock, Rong Yuan, Alfred S. Lewin, and Gregory S. Schultz
9 Control of the Interferon Response in RNAi Experiments; Jana Nejepinska, Matyas Flemr, and Petr Svoboda
10 shRNA-induced Interferon-Stimulated Gene Analysis; Nuria Morral and Scott R. Witting
11 Use of RNA Interference to Investigate Cytokine Signal Transduction in Pancreatic Beta Cells; Fabrice Moore, Daniel. A. Cunha, Hindrik Mulder, and Decio L. Eizirik
12 Ligand Affinity Chromatography, an Indispensable Method for the Purification of Soluble Cytokine Receptors and Binding Proteins; Daniela Novick and Menachem Rubinstein
13 In Vitro Stimulation and Detection of IFNa Production in Human Plasmacytoid Dendritic Cells; William C. Adams and Karin Lore
14 A Sensitive and Versatile Cytokine Bioassay Based on Type I interferon Signaling in 2fTGH Cells; Lennart Zabeau, Jose Van der Heyden, and Jan Tavernier

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