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This fully updated edition includes the novel identification, detection, and typing technologies that are transforming the field of diagnostic testing. The authors, working at the forefront of diagnostic test development, highlight these new technologies and describe current and future molecular diagnostic tests and related nucleic acid extraction methods. The protocols range from advanced molecular detection, quantification, and typing systems, to protocols for diagnostic protein identification, serological testing, and cell culture-based assays. The emphasis is on nucleic acid-based diagnostics and alternative biochemically- and immunologically-based formats that can provide significant potential for multiparameter testing and automation.
There are several unique features of this book. First, it deals exclusively with bacteria. Second, it should prove useful in a wide rang of techniques are described. - Doody's Health Sciences Book Review Journal
Antibiotic Resistance Profiling. Bacteriocin Typing. Bacteriophage Typing. The Analysis of Bacterial Proteins by SDS Polyacrylamide Gel Electrophoresis. Lipopolysaccharide Chemotyping. Analysis of Bacterial Outer Membrane Proteins. Multilocus Enzyme Electrophoresis. Fast Atom Bombardment-Mass Spectrometry. Pyrolysis Mass Spectrometry. The Direct Immunofluorescent Filter Technique (DIFT). The Direct Epifluorescent Filter Technique (DEFT). Use of Commercially Available ELISA Kits for the Detection of Foodborne Pathogens. Detection of Foodborne Pathogens Using DNA Probes and a Dipstick Format. Preparation of Bacterial Genomic DNA. Pulsed Field Gel Electrophoresis. Application of Total DNA Restriction Pattern Analysis to Identification and Differentiation of Bacterial Strains. Determination of rRNA Gene Restriction Patterns. Preparation of Nonradioactive DNA Probes. Preparation of Bacterial Plasmid DNA. Plasmid Profile Typing and Plasmid Fingerprinting. Polymerase Chain Reaction for the Detection of Listeria Species and Listeria Monocytogenes. Development of Bacterial Species-Specific DNA Probes Based on Ribosomal RNA Genes Using PCR. Identification of Microorganisms Using Random Primed PCR. Ligase Chain Reaction. Index.
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