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About this book
About this book
A collection of detailed molecular methods for the environmental monitoring of bacterial populations. Methods include techniques for the recovery of cells, for the analysis of whole cells, and for the molecular study of target species and heterogeneous populations.
Some Problems Posed by Natural Environments for Monitoring Microorganisms C. Edwards. Sampling Sediment and Soil: Use of Coring Devices R.Pickup G. Rhodes and G. Hall. Sampling Water Bodies: Tangential Flow Filtration R. Pickup H. Mallinson and G. Rhodes. Dielectrophoresis D.W.Pimbley P.D. Patel and C.J. Robertson. Flow Cytometry and Cell Sorting: Rapid Analysis of Individual Cells from Natural Environments J. Porter. Magnetic Particle Based Separation Techniques for Monitoring Bacteria from Natural Environments J. Porter. DNA Extraction from Natural Environments K. Bruce P. Strike and D. A. Ritchie. Automated Sequencing of DNA Retrieved from Environmental Samples M. Upton. Analysis of DNA SequencesM. Upton. Fluorescent PCR/RFLP Monitoring of Genes Amplified Directly from Bacterial Communities in Soils and Sediments K.D. Bruce and M.R.Hughes. Recovery and Analysis of Ribosomal RNA Sequences from the Environment I.M. Head. Application of DGGE (denaturing gel electrophoresis) to Microbial Ecology R. Hastings. Reporter Genes Glover. Approaches to Characterizing Microorganisms in the Environment by Fatty Acid Analysis I.P.Thompson M.J.Bailey and A.K.Lilley. Fluorescent in situ hybridization and the Analysis of the Single Cell A.G.O'Donnell and A.S. Whiteley. Specific Detection Viability Assessment and Macromolecular Staining of Bacteria for Flow Cytometry J. Porter. Confocal Laser Scanning Microscopy of Environmental Samples D. Lloyd A.J. Hayes B.Hill L. Durrant and J.R. Ralphs. Monitoring Microbial Activities Using Membrane Inlet Mass Spectrometry J.R.Firth and C. Edwards. Experimental Biofilms and Their Applications in the Study of Environmental Processes J.C. Rayner and H. Lappin-Scott. Establishment of Experimental Biofilms Using the Modified Robbins Device and Flow Cells L.Hall-Stoodley J.C.Rayner P. Stoodley and H.Lappin-Scott.
Handbook / Manual
333 pages, B/w illus, figs, diags, tabs
This book looks at state-of-the-art techniques for monitoring bacteria in natural environments, offers step-by-step instructions to ensure readily reproducible results, and provides numerous tips on methods for optimizing results. - Environmental Science & Technology, July 1, 1999 All contributions conformed to a given structure and so the individual sections have turned out to be compact as well as comprehensive and clear. They describe the principles of different techniques as well as their applications and limitations and most of them contain easy-to-follow protocols providing immediate access to a given method. - International Journal of Hygiene and Environmental Health