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A collection of readily reproducible classic and emerging molecular methods for the laboratory isolation and identification of the pathogens, viruses, and parasites that cause food-borne disease. Among the pathogens covered are specific bacteria, including Salmonella spp, Campylobacter spp., Listeria spp., and Bacillus spp.; viruses, including noroviruses and enteroviruses; and parasites, including Cryptosporidium and seafood nematode worms. The protocols follow the successful Methods in BiotechnologyT series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
The Bacteria; Detection of Hemolysins in Aeromonas spp. Isolates from Food Sources: PCR Analysis and Biological; Activity; Rosabel Falcon, Tatiana d'Albuquerque de Castro, Maria das Gracas de Luna, Angela Correa de; Freitas-Almeida and Tomomasa Yano; Detection and Purification of Bacillus Cereus Enterotoxins; Toril Lindback and Per Einar Granum; Campylobacter: Isolation, Identification and Preservation; Rachel Gorman and Catherine C. Adley; Detection of Clostridium Botulinum by Multiplex PCR in Foods and Feces; Miia Lindstrom, Mari Nevas and Hannu Korkeala; Multiplex PCR for the Specific Identification of Enterohemorrhagic Escherichia coli Strains in the; O157:H7 Complex; Peter C.H. Feng and Steven R. Monday; PulseNet's Step-by-Step Laboratory Protocol for Molecular Subtyping of Listeria Monocytogenes by; Macrorestriction and Pulsed-Field Gel Electrophoresis; Lewis M. Graves and Balasubramanian Swaminathan; Plesiomonas Shigelloides: Detection by PCR; Ivan Ciznar, Carlos Gonzales-Rey, Karel Krovacek and Anna Hostacka; Pulsed Field Gel Electrophoresis as a Molecular Technique in Salmonella Epidemiological Studies; Rachel Gorman and Catherine C. Adley; Kits for the Detection of Food Poisoning Toxins Produced by Bacillus Cereus and Staphylococcus; Aureus; Moira M. Brett; Microbiological and Molecular Methods to Identify and Characterize Toxigenic Vibrio Cholerae from; Food Samples; Keya De, Ranjan K Nandy and G Balakrish Nair; HPLC Measurement of Aflatoxin B1 and Metabolites in Isolated Rat Hepatocytes; Jennifer Colford; The Viruses; Detection of Noroviruses of Genogroups I and II in Drinking Water by Real-Time One-Step RT-PCR; (Reverse Transcription Polymerase Chain-Reaction); Christian M. Beuret. Detection of Enteroviruses; Miguel-Angel Jimenez-Clavero, Victoria Ley, Nuria Gomez, and Juan-Carlos Saiz; Detection of Hepatitis A Virus and Rotavirus Using Nucleic Acid Sequence-Based Amplification; (NASBA); Julie Jean and Ismail Fliss; The Protozoa; The Isolation and Characterisation of Cathepsin L1 Protease from Fasciola Hepatica; Excretory-Secretory Products for the Serodiagnosis of Human Fasciolosis; Sandra O'Neill, Grace Mulcahy and John Dalton; Molecular Biology Methods for Detection and Identification of Cryptososporidium Species in Faeces,; Water and Shell Fish; C.J P. Lowery, L. Xiao, U.M. Ryan, J.S. Dooley, B.C. Millar and J.E. Moore; Molecular Identification of Nematode Worms from Seafood (Anisakis spp. and Pseudoterranova spp); and Meat (Trichinella spp.); Giuseppe La Rosa, Stefano D'Amelio and Eduardo Pozio; Parallel Studies to the Analysis of Food Borne Pathogens; Approaches to Developing Quantitative Risk Assessment Models; Enda J. Cummins; A Review of Surveillance Networks of Foodborne Diseases; Camelia Molnar, Rita Wels and Catherine C Adley.