A distinguished panel of international experts reviews the importance of glycobiology, an interdisciplinary field encompassing chemistry, biochemistry, biology, physiology, and pathology. Glycobiology Protocols highlights important methodological progress that has been made in the field of glycobiology, helping scientists to answer specific questions on glycoprotein structures, on the biosynthesis of glycoconjugates, and on the functions of lipid- or protein-bound carbohydrates. Glycobiology Protocols contains specific methods for the analysis of the structures or functions of glycoconjugates, as well as of glycosyltransferases and glycosidases involved in the biosynthesis of glycans. The methods described for specific systems can be modified for investigations of similar biomolecules and tissues or cell types. Comprehensive and highly practical, Glycobiology Protocols provides researchers with a solid basis for studying the many aspects of glycobiology with state-of-the-art methods. The protocols follow the successful Methods in Molecular BiologyT series format, with each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
The Application of Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) for the Study of the Dolichol Pyrophosphate-Linked Oligosaccharides Pathway in Cell Cultures and Animal Tissues; Ningguo Gao; Partial Purification of Mannosylphosphorylundecaprenol Synthase from Micrococcus luteus: A Useful Enzyme for the Biosynthesis of a Variety of Mannosylphosphorylpolyisoprenol Products; Jeffrey S. Rush and Charles J. Waechter; Assay of Dolichyl-Phospho-Mannose Synthase Reconstituted in a Lipid Matrix; John S. Schutzbach; O-Mannosylation in Mammalian Cells; Tamao Endo and Hiroshi Manya; Methods for Analysis of Unusual Forms of O-glycosylation; Aleksandra Nita-Lazar and Robert S. Haltiwanger; Structural Analysis of Naturally Occurring Sialic Acids; Johannis P. Kamerling and Gerrit J. Gerwig; Non-Radioactive Trans-Sialidase Screening Assay; Silke Schrader and Roland Schauer; Structural Determination of O-Glycans by Tandem Mass Spectrometry; Catherine Robbe, Jean-Claude Michalski, and Calliope Capon; Detailed Structural Analysis of N-glycans Released from Glycoproteins in SDS-PAGE Gel Bands Using HPLC Combined with Exoglycosidase Array Digestions; Louise Royle, Catherine M. Radcliffe, Raymond A. Dwek, and Pauline M. Rudd; Molecular Modelling of Glycosyltransferases; Anne Imberty, Michaela Wimmerova, Jaroslav Koca, and Christelle Breton; b-Galactoside a2,6 Sialyltransferase (ST6Gal.I) and the Sialyl a2,6-galactosyl-linkage in Tissues and Cell Lines; Fabio Dall'Olio, Nadia Malagolini, and Mariella Chiricolo; Visualizing Intracellular Distribution and Activity of Core 2 b(1,6)N-acetylglucosaminyltransferase-I (C2GnT-I) in Living Cells; Maelle Prorok-Hamon, Sylvie Mathieu, and Assou El-Battari; Gene Expression Analysis of Glycosylation-Related Genes by Real-Time PCR; Juan J. Garcia-Vallejo, Sonja I. Gringhuis, Willem van Dijk, and Irma van Die; Analysis of the Glycodynamics of Primary Osteoblasts and Bone Cancer Cells; Inka Brockhausen, Xiaojing Yang, and Mark Harrison; Micromethods for the Characterization of Lipid A-Core and O Antigen Lipopolysaccharide; Cristina L. Marolda, Piya Lahiry, Enrique Vines, Soledad Saldias, and Miguel A. Valvano; Assays for a Galactosyltransferase Involved in the Assembly of the O7 Antigen Repeat Unit of Escherichia coli; Inka Brockhausen, Walter A. Szarek, John G. Riley, and Jason Vlahakis; High Through-Put Quantitation of Metabolically Labeled Anionic Glycoconjugates by Scintillation Proximity Assay Utilizing Binding to Cationic Dyes; Karen J. Rees-Milton and Tassos P. Anastassiades; Quantitative Analysis of Mucins in Mucosal Secretions Using Indirect ELISA; Pablo Argueso and Ilene K. Gipson; Molecular Dissection of the Mouse Zona Pellucida: An Electron Microscopic Perspective Utilizing High Resolution Colloidal-Gold Labeling Methods; Frederick W.K. Kan; Soluble Adamantly GSL Analogues as Probes of GSL Function; Clifford Lingwood, Skanda Sadacharan, Maan Abul-Milh, Murugespillai Mylvaganum, and Marcus Peter; Diagnosis of Krabbe Disease by Use of a Natural Substrate; John W. Callahan and Marie-Anne Skomorowski; In Vitro Assays of the Functions of Calnexin and Calreticulin, Lectin-Chaperones of the Endoplasmic Reticulum; Breanna S. Ireland, Monika Niggemann, and David B.Williams; Quantitative Measurement of Selectin-Ligand Interactions: Assays to Identify a Sweet Pill in a Library of Carbohydrates; Mark E. Beauharnois, Sriram Neelamegham, and Khushi L. Matta; Sialic Acid Binding Specificities of Siglecs; Nadine Bock and Soerge Kelm
...a very valuable handbook for a wide audience of specialists involved in studies of carbohydrates and carbohydrate-containing biopolymers; glycobiologists, biochemists, bioorganic chemists, and molecular and cell biologists. -Biochemistry