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High content screening (HCS) was developed by Cellomics Inc. in the mid-1990s to address the need for a platform that could be used in the discovery-driven research and development required to understand the functions of genes and gene products at the level of the cell. High Content Screening: A Powerful Approach to Systems Cell Biology and Drug Discovery discusses its use as a high throughput platform to understand the functions of genes, RNA, proteins, and other cellular constituents at the level of the living cell. High Content Screening is assembled to assist both existing users of HCS, as well as investigators considering the addition of a discovery-driven platform to their research and development activities. The chapters have been organized into sections that highlight the importance of integrating instrumentation, application software, reagents, and informatics. In addition, there is a combination of pure review chapters on key topics and specific methods chapters. Written by a team of world leaders in the field, High Content Screening is essential reading for senior scientists, advanced lab technicians, as well as students/post-docs who wish to apply HCS in their research and development programs.
Table of Contents; High Content Screening: A Powerful Approach to Systems Cell Biology and Drug Discovery; Editors: D. Lansing Taylor1, Jeffrey R. Haskins2 and Kenneth A. Giuliano1; 1Cellumen, Inc. and 2Cellomics, Inc.; I. Introduction to High Content Screening; A. Past, Present and Future of HCS and the Field of Cellomics [Review Chapter]; D. Lansing Taylor; B. A Pharmaceutical Company User's Perspective on the Potential of High Content Screening (HCS) in Drug Discovery [Review Chapter]; Ann F. Hoffman, B.S. and Ralph J. Garippa; C. Linking Microscopy and High Content screening in Large-scale Biomedical Research [Review Chapter]; James G. Evans and Paul Matsudaira; II. Instrumentation, Biological Application Software, and Sample Preparation; A. Requirements, Features and Performance of High Content Screening Platforms [Review Chapter]; Albert H. Gough and Paul A. Johnston; B. Characteristics & Value of Directed Algorithms in HCS [Review Chapter]; Richik N. Ghosh, Oleg Lapets and Jeffrey R. Haskins; C. Characteristics and Value of Machine Learning for Imaging in HCS [Review Chapter]; Jurgen A. Klenk; D. Tools for Quantitative and Validated Measurements of Cells; Anne L. Plant, John T. Elliott, Alessandro Tona, Dennis McDaniel, and Kurt J. Langenbach; E. Automated Cell Plating and Sample Treatments for Fixed Cells in High Content Assays; Peter B. Simpson and Gillian R. Richards; F. Differentiating Primary Human Cells in Rapid-Throughput Discovery Applications; Daniel R. Marshak and Dale E. Greenwalt; G. Use of the CellCard[trademark] System for Analyzing Multiple Cell Types in Parallel; Oren Beske, Daniel Bassoni, and Simon Goldbard; III. Reagents; A. Reagents to measure and manipulate cell functions [Review Chapter]; Kenneth A. Giuliano, D. Lansing Taylor, and Alan S. Waggoner; B. Fluorescent Proteins and Engineered Cell Lines [Review Chapter]; Nick Thomas; C. Optimizing the integration of immunoreagents and fluorescent probes for multiplexed HCS assays; Kenneth A. Giuliano; D. The HaloTag[trademark]: A Novel Technology for Cell Imaging and Protein Analysis; Georgyi V. Los and Keith Wood; E. Protein labeling with FlAsH and ReAsH; Thomas Machleidt, Matt Roberts, and George Hanson. F. Exploiting Network Biology to Improve Drug Discovery [Review Chapter]; Marnie L. MacDonald and John K. Westwick; G. Physiological Indicators of Cell Function [Review Chapter]; Michael J. Ignatius and Jeffrey Hung; H. The Use of siRNA to Validate Immunofluorescence Studies; K. Gregory Moore, Wayne Speckmann, and Ron Herzig; I. Caged Substrates Applied to High Content Screening: An Introduction with an Eye to the Future; Peter G. Conrad, II, Rajesh V. Chavli, and Richard S. Givens; IV. Informatics and Bioinformatics; A. Overview of Informatics for HCS [Review Chapter]; R. Terry Dunlay, Wallace J. Czekalski, and Mark A. Collins; B. Large-Scale Data Management for High Content Screening [Review Chapter]; Leon S. Garfinkel; C. An Integrated Biomedical Knowledge Extraction and Analysis Platform: Using Federated Search and Document Clustering Technology; Donald P. Taylor; D. Visualization of High Content Screening Data [Review Chapter]; Matthew J. Anstett; E. Pathway Mapping Tools For Analysis of High Content Data [Review Chapter]; Sean Ekins, Yuri Nikolsky, Andre Bugrim, Eugene Kirillov, and Tatiana Nikolskaya; V. Assays and Applications of HCS; A. Systems Biology in Cancer Research: Genomics to Cellomics; Jackie L. Stilwell, Yinghui Guan, Richard M. Neve, and Joe W. Gray; B. Target Validation in Drug Discovery; Robert A. Blake; C. HCS as a Screening Tool in Drug Discovery; Anthony Nichols; D. Discovery of Protein Kinase Phosphatase Inhibitors; Andreas Vogt and John S. Lazo; E. High Content Translocation Assays for Pathway Profiling [Review Chapter]; Frosty Loechel, Sara Bjorn, Viggo Linde, Morten Praestegaard, and Len Pagliaro; F. In vitro Cytotoxicity Assessment [Review Chapter]; Peter O'Brien and Jeffrey R. Haskins; G. Neurite Outgrowth in Retinal Ganglion Cell Culture; John B. Kerrison and Donald J. Zack.