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Human Retrovirus Protocols: Virology and Molecular Biology

Handbook / Manual

Series: Methods in Molecular Biology Volume: 304

By: Tuofu Zhu

Humana Press

Hardback | Dec 2005 | #163644 | ISBN: 1588294951
Availability: Usually dispatched within 1 week Details
NHBS Price: £129.99 $169/€146 approx

About this book

A cutting-edge collection of basic and state-of-the-art methods optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from methods for the isolation and detection of human retroviruses to cutting-edge methods for exploring the interplay between the viruses and the host. Here, the researcher will find up-to-date techniques for the isolation and propagation of HIV, HTLV, and foamy virus from a variety of sources. There are also assays for determining the cell tropism of HIV-1, the coreceptor usage of HIV-1, and human gene expression with HIV-1 infection by microarrays, as well as for phenotyping HIV-1 infected monocytes and examining their fitness. Highlights include the detection and quantification of HIV-1 in resting CD4+, a new cloning system for making recombinent virus, cDNA microarrays, and the determination of genetic polymorphisms in two recently identified HIV-1 co-factors that are critical for HIV-1 infection.


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