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Macromolecular Crystallography Protocols, now in two volumes, examines major developments that have occurred since publication of the acclaimed first edition nearly a decade ago. Volume 1, Preparation and Crystallization of Macromolecules and Volume 2, Structure Determination, explore recent advances that have accelerated the pace of structural determination and made crystallography accessible to a broader range of investigators. Volume 1 is composed of detailed protocols for the preparation and optimization of crystals, including tips from the experts on the best methods for inducing proteins to adopt their crystalline form. Volume 2 complements the first volume by addressing laboratory techniques for crystal handling and structural characterization, as well as computational techniques for data collection, phasing, and refinement. The volume concludes with a detailed and insightful survey of available crystallographic software. These volumes will be an indispensable reference for obtaining macromolecular crystals and determining their three-dimensional structure.
TABLE OF CONTENTS VOLUME 1; HUMANA PRESS-CRYSTALLOGRAPHIC METHODS AND PROTOCOLS; Sylvie Doublie, Editor; CHAPTER TITLE AUTHOR(S) PAGE; 1. A Generic Method for the Production of Recombinant Proteins in Escherichia coli Using a Dual His6-MBP Affinity Tag; Joseph E. Tropea, Scott Cherry, Sreedevi Nallamsetty, Christophe Bignon, and David S.Waugh; 1-29; 2. Cloning, production, and purification of proteins for a medium scale structural genomics project Sophie Quevillon-Cheruel, Bruno Collinet, Lionel Tresaugues, Philippe Minard, Gilles Henckes, Robert Aufrere, Karine Blondeau, Cong-Zhao Zhou, Dominique Liger, Nabila Bettache, Anne Poupon, Ilham Aboulfath, Nicolas Leulliot, Joel Janin, and Herman van Tilbeurgh; 1-26; 3. Baculoviral expression of an integral membrane protein for structural studies; Dean R. Madden and Markus Safferling 1-33; 4. Protein Engineering Sonia Longhi, Francois Ferron, and Marie-Pierre Egloff; 1-48; 5. Selenomethionyl protein expression Sylvie Doublie 1-21; 6. How to Use Dynamic Light Scattering to Improve Your Chances of Growing Crystals of a Macromolecule; Gloria Borgstahl 1-34; 7. Screening and optimization methods for non-automated crystallization laboratories; Terese Bergfors 1-32; 8. Improving Marginal Crystals Charles W. Carter, Jr. and; Madeleine Ries-Kautt; 1-34; 9. Optimisation Techniques for Automation and High Throughput; Naomi E. Chayen 1-26; 10. Three-dimensional crystallization of membrane proteins; James Fethiere 1-40; 11. Crystallization of Protein-DNA complexes; Thomas Hollis 1-20; 12. Preparation and crystallization of RNA; Barbara Golden 1-24; 13. Crystallisation of RNA-protein complexes Eiji Obayashi, Chris Oubridge, Daniel Pomeranz Krummel, and Kiyoshi Nagai; 1-27