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Academic & Professional Books  Organismal to Molecular Biology  Biochemistry & Molecular Biology

Macromolecular Crystallography Protocols Volume I: Preparation and Crystallization of Macromolecules

Handbook / Manual
Series: Methods in Molecular Biology Volume: 363
Edited By: Sylvie Doublie
312 pages
Publisher: Humana Press
Macromolecular Crystallography Protocols
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  • Macromolecular Crystallography Protocols ISBN: 9781588292926 Edition: 2 Hardback Feb 2007 Not in stock: Usually dispatched within 1-2 weeks
Price: £89.99
About this book Contents Customer reviews Related titles

About this book

Macromolecular Crystallography Protocols, now in two volumes, examines major developments that have occurred since publication of the acclaimed first edition nearly a decade ago. Volume 1, Preparation and Crystallization of Macromolecules and Volume 2, Structure Determination, explore recent advances that have accelerated the pace of structural determination and made crystallography accessible to a broader range of investigators. Volume 1 is composed of detailed protocols for the preparation and optimization of crystals, including tips from the experts on the best methods for inducing proteins to adopt their crystalline form. Volume 2 complements the first volume by addressing laboratory techniques for crystal handling and structural characterization, as well as computational techniques for data collection, phasing, and refinement. The volume concludes with a detailed and insightful survey of available crystallographic software. These volumes will be an indispensable reference for obtaining macromolecular crystals and determining their three-dimensional structure.


TABLE OF CONTENTS VOLUME 1; HUMANA PRESS-CRYSTALLOGRAPHIC METHODS AND PROTOCOLS; Sylvie Doublie, Editor; CHAPTER TITLE AUTHOR(S) PAGE; 1. A Generic Method for the Production of Recombinant Proteins in Escherichia coli Using a Dual His6-MBP Affinity Tag; Joseph E. Tropea, Scott Cherry, Sreedevi Nallamsetty, Christophe Bignon, and David S.Waugh; 1-29; 2. Cloning, production, and purification of proteins for a medium scale structural genomics project Sophie Quevillon-Cheruel, Bruno Collinet, Lionel Tresaugues, Philippe Minard, Gilles Henckes, Robert Aufrere, Karine Blondeau, Cong-Zhao Zhou, Dominique Liger, Nabila Bettache, Anne Poupon, Ilham Aboulfath, Nicolas Leulliot, Joel Janin, and Herman van Tilbeurgh; 1-26; 3. Baculoviral expression of an integral membrane protein for structural studies; Dean R. Madden and Markus Safferling 1-33; 4. Protein Engineering Sonia Longhi, Francois Ferron, and Marie-Pierre Egloff; 1-48; 5. Selenomethionyl protein expression Sylvie Doublie 1-21; 6. How to Use Dynamic Light Scattering to Improve Your Chances of Growing Crystals of a Macromolecule; Gloria Borgstahl 1-34; 7. Screening and optimization methods for non-automated crystallization laboratories; Terese Bergfors 1-32; 8. Improving Marginal Crystals Charles W. Carter, Jr. and; Madeleine Ries-Kautt; 1-34; 9. Optimisation Techniques for Automation and High Throughput; Naomi E. Chayen 1-26; 10. Three-dimensional crystallization of membrane proteins; James Fethiere 1-40; 11. Crystallization of Protein-DNA complexes; Thomas Hollis 1-20; 12. Preparation and crystallization of RNA; Barbara Golden 1-24; 13. Crystallisation of RNA-protein complexes Eiji Obayashi, Chris Oubridge, Daniel Pomeranz Krummel, and Kiyoshi Nagai; 1-27

Customer Reviews

Handbook / Manual
Series: Methods in Molecular Biology Volume: 363
Edited By: Sylvie Doublie
312 pages
Publisher: Humana Press
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