Microbial Metabolic Engineering Methods and Protocols

About this book

Metabolic engineering is the practice of genetically optimizing metabolic and regulatory networks within cells to increase production and/or recovery of certain substance from cells. In Microbial Metabolic Engineering: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study metabolic engineering. These include methods and techniques to engineer genes and pathways, use of modern biotechnology tools in microbial metabolic engineering, and examples of metabolic engineering for real world applications such as whole cell biosensors and acetate control in large scale fermentation.

Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microbial Metabolic Engineering: Methods and Protocols seeks to provide researchers with an overview of key topics on microbial metabolic engineering.

Contents

Preface
Preamble

1 Screening for Cellulases with Industrial Value and their use in Biomass Conversion Julia Jurgensen, Nele Ilmberger and Wolfgang R. Streit
2 Reversal of NAD(P)H Cofactor Dependence by Protein Engineering Sabine Bastian and Francis H. Arnold
3 Quantifying Plasmid Copy Number to Investigate Plasmid Dosage Effects Associated with Directed Protein Evolution Samuel Million-Weaver, David L. Alexander, Jennifer M. Allen, and Manel Camps
4 High Isoprenoid Flux Escherichia coli as a Host for Carotenoids Production Wonchul Suh
5 Mutagenic Inverted Repeats Assisted Genome Engineering (MIRAGE) in Saccharomyces cerevisiae: Deletion of gal7 Nikhil U. Nair and Huimin Zhao
6 Creation of New Metabolic Pathways or Improvement of Existing Metabolic Enzymes by in vivo Evolution in Escherichia coli Isabelle Meynial-Salles and Philippe Soucaille
7 Bioluminescent Reporter Genes for Promoter Discovery Tina K. Van Dyk
8 Recombination-based DNA Assembly and Mutagenesis Methods for Metabolic Engineering Xiquan Liang, Lansha Peng, Billyana Tsvetanova, Ke Li, Jian-Ping Yang, Tony Ho, Josh Shirley, Liewei Xu, Jason Potter, Wieslaw Kudlicki, Todd Peterson, and Federico Katzen
9 Ethanol-tolerant Gene Identification in Clostridium thermocellum using Pyro-resequencing for Metabolic Engineering Shihui Yang, Dawn M. Klingeman, and Steven D. Brown
10 Use of Proteomic Tools in Microbial Engineering for Biofuel ProductionShaomin Mao, Kaizhi Jia, Yanping Zhang, Yin Li
11 Metabolic Engineering of Antibiotic-producing Actinomycetes using in vitro Transposon Mutagenesis Andrew R. Reeves and J. Mark Weber
12 Use FACS Sorting in Metabolic Engineering of Escherichia coli for Increased Peptide Production Qiong Cheng, Kristin Ruebling-Jass, Jianzhong Zhang, Qi Chen and Kevin Croker
13 Using Flux Balance Analysis to Guide Microbial Metabolic Engineering Kathleen A. Moran, Nathan C. Crook and Hal S. Alper
14 Using an Advanced Microfermentor System for Strain Screening and Fermentation Optimization Dongming Xie
15 Rapid Strain Evaluation using Dynamic DO-stat fed-batch Fermentation Under Scale-down Conditions Jun Sun
16 Preparation and Evaluation of Lignocellulosic Biomass Hydrolysates for Growth by Ethanolgenic Yeasts Ying Zha, Ronald Slomp, Johan van Groenestijn and Peter Punt
17 Engineering Whole-cell Biosensors with No Antibiotic Markers for Monitoring Aromatic Compounds in the Environment Aitor de las Heras and Victor de Lorenzo
18 Metabolic Engineering for Acetate Control in Large Scale Fermentation Yong Tao, Qiong Cheng and Alexander D, Kopatsis
19 Minimization and Prevention of Phage Infections in Bioprocesses Marcin Los Shaomin Mao, Kaizhi Jia, Yanping Zhang, Yin Li

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