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Hands-on experts in nanomaterial synthesis and application describe in detail the key experimental techniques currently employed in novel materials synthesis, dynamic cellular imaging, and biological assays. The author's emphasize diverse strategies to synthesize and functionalize the use of nanoparticles for biological applications. Additional chapters focus on the use of biological components (peptides, antibodies, and DNA) to synthesize and organize nanoparticles to be used a building block in larger assemblies. These new materials make it possible to image cellular processes for longer durations, leading to high throughput cellular-based screens for drug discovery, drug delivery, and diagnostic applications. Highlights include overview chapters on quantum dots and DNA nanotechnology, and cutting-edge techniques in the emerging nanobiotachnology arena.
1 Applications of Quantum Dots in Biology: An Overview Charles Z. Hotz 2 Fluoroimmunoassays Using Antibody-Conjugated Quantum Dots Ellen R. Goldman, Hedi Mattoussi, George P. Anderson, Igor L. Medintz, and J. Matthew Mauro 3 Labeling Cell Surface Proteins Via Antibody Quantum Dot Streptavidin Conjugates John Mason, Ian D. Tomlinson, Sandra J. Rosenthal, and Randy Blakely 4 Peptide-Conjugated Quantum Dots: Imaging the Angiotensin Type 1 Receptor in Living Cells Ian D. Tomlinson, John Mason, Randy Blakely, and Sandra J. Rosenthal 5 Quantum Dot-Encoded Beads Xianhu Gao and Shuming Nie 6 The Use of Nanobarcodes (R) Particles in Bioassays R. Griffith Freeman, Paul A. Raju, Scott M. Norton, Ian D. Walton, Patrick C. Smith, Lin He, Michael J. Natan, Michael Y. Sha and Sharron G. Penn 7 Assembly and Characterization of Biomolecule Gold Nanoparticle Conjugates and Their Use in Intracellular Imaging Alexander Tkachenko, Wilhelm R. Glomm, Joeseph Ryan, Huan Xie, Stefan Franzen, and Daniel Feldheim 8 A Whole Blood Immunoassay Facilitated by Gold Nanoshell-Conjugate Antibodies Lee R. Hirsch, Naomi J. Halas, and Jennifer L. West 9 Assays for the Selection of ScFv Recombinant Antibodies to Metal Nanoclusters Jennerifer Edl, Ray Mernaugh, and David W. Wright 10 Surface Functionalized Nanoparticles for Controlled Drug Delivery Sung-Wook Choi, Woo-Sik Kim, and Jung-Hyun Kim 11 Screening of Combinatorial Peptide Libraries for Nanocluster Synthesis Joeseph M. Slocik and David W. Wright 12 Structural DNA Nanotechnology: An Overview Nadrian C. Seeman 13 Nanostructured DNA Templates Jeffery L. Coffer, Russell F. Pinizzotto, and Young Gyu Rho 14 Probing DNA Structure with Nanoparticles Rahina Mahtab and Catherine J. Murphy 15 Synthetic Nanoscale Elements for the Delivery of Materials into Viable Cells Timothy. E. McKnight, Anatoli V. Melechko, Michael A. Guillorn, Vladimir I. Merkulov, Douglas H. Lowndes, and Michael L. Simpson 16 Real-Time Cell Dynamics with a Multianalyte Physiometer Sven E. Eklund, Eugene Kozlov, Dale E. Taylor, Franz Baudenbacher, and David E. Cliffel