This volume is dedicated entirely to the characterization of peptides and their application for study of biochemical systems. The works presented describe the most recent advances in analytical techniques, as well as the systematic manner in which peptides can be constructed, analyzed, and applied to attack contemporary problems in biochemistry.
Peptide Characterization and Application Protocols Preface Contributors Section 1: Characterization Chapter 1: HPLC Analysis and Purification of Peptides Colin T. Mant, Yuxin Chen, Zhe Yan, Traian V. Popa, James M. Kovacs, Janine B. Mills, Brian P. Tripet, and Robert S. Hodges Chapter 2: Identification of Proteins Based on MS/MS Spectra and Location of Post-translational Modifications Kathryn L. Stone, Myron Crawford, Walter McMurray, Nancy Williams, and Kenneth R. Williams Chapter 3: Determination of Phosphorylated and O-glycosylated Sites by Chemical Targeting (CTID) at Ambient Temperature Gary M. Hathaway Chapter 4: Synthesis, Biosynthesis, and Characterization of Transmembrane Domains of a G Protein-Coupled Receptor Fred Naider Section 2: Application Chapter 5: Application of Topologically Constrained Mini-Proteins as Ligands, Substrates, and Inhibitors Janelle L. Lauer-Fields, Dmitriy Minond, Keith Brew, and Gregg B. Fields Chapter 6: Proteolytic Profiling of the Extracellular Matrix Degradome Diane Baronas-Lowell, Janelle L. Lauer-Fields, Mohammad Al-Ghoul, and Gregg B. Fields Chapter 7: A -Amyloid Protein Aggregation Inhibition Marcus A. Etienne, Nadia J. Edwin, Jed P. Aucoin, Paul S. Russo, Robin L. McCarley, and Robert P. Hammer Chaper 8: Radiometal-labeled Somatostatin Analogues for Applications in Cancer Imaging and Therapy Jason S. Lewis and Carolyn J. Anderson Chapter 9: Cell Penetrating Proline Rich Peptidomimetics Josep Farrera-Sinfreu, Ernest Giralt, Miriam Royo, and Fernando Albericio Chapter 10: Peptide-Mediated Targeting of Liposomes to Tumor Cells Evonne M. Rezler, David R. Khan, Raymond Tu, Matthew Tirrell, and Gregg B. Fields Chapter 11: Peptide-Mediated Delivery of Nucleic Acids into Mammalian Cells Sebastien Deshayes, Federica Simeoni, May C. Morris, Gilles Divita, and Frederic Heitz Chaper 12: Broth Microdilution Antibacterial Assay of Peptides Laszlo Otvos and Mare Cudic Chapter 13: Depsipeptide Synthesis Maciej Stawikowski and Predrag Cudic