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Academic & Professional Books  Organismal to Molecular Biology  Biochemistry & Molecular Biology

Peptide Synthesis and Applications

Handbook / Manual
Series: Methods in Molecular Biology Volume: 298
By: John Howl
Publisher: Humana Press
Peptide Synthesis and Applications
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  • Peptide Synthesis and Applications ISBN: 9781588293176 Hardback Dec 2005 Usually dispatched within 1-2 weeks
    £119.99
    #163677
Price: £119.99
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About this book

Hands-on experts describe in step-by-step detail the key methodologies of contemporary peptide synthesis and illustrate their numerous applications. The techniques presented include protocols for chemical ligation, the synthesis of cyclic and phosphotyrosine-containing peptides, lipoamino acid- and sugar-conjugated peptides, and peptide purification and analyses. Additional chapters detail methodologies and instrumentation for high-throughput peptide synthesis, many different applications of peptides as novel research tools and biological probes, and the design and application of fluorescent substrate-based peptides that can be used to determine the selectivity and activity of peptidases. A practical guide to the identification of proteins using mass spectrometric analyses of peptide mixtures is also included.

Contents

Part I: Common Strategies 1 Fundamentals of Modern Peptide Synthesis Muriel Amblard, Jean-Alain Fehrentz, Jean Martinez, and Gilles Subra 2 Chimerism: A Strategy to Expand the Utility and Applications of Peptides John Howl Part II: Synthetic Methodologies and Applications 3 Modification of Peptides and Other Drugs Using Lipoamino Acids and Sugars Joanne T. Blanchfield and Istvan Toth 4 Synthesis of Linear, Branched, and Cyclic Peptide Chimera Gabor Mezo and Ferenc Hudecz 5 Synthesis of Cell-Penetrating Peptides for Cargo Delivery Margus Pooga and Ulo Langel 6 Incorporation of the Phosphotyrosyl Mimetic 4-(Phosphonodifluormethyl)phenylalanine (F2Pmp) into Signal Transduction-Directed Peptides Zhu-Jun Yao, Kyeong Lee, and Terrence R. Burke, Jr. 7 Expressed Protein Ligation for Protein Semisynthesis and Engineering Zuzana Machova and Annette G. Beck-Sickinger 8 Cellular Delivery of Peptide Nucleic Acid by Cell-Penetrating Peptides Kalle Kilk and Ulo Langel 9 Quenched Fluorescent Substrate-Based Peptidase Assays Rebecca A. Lew, Nathalie Tochon-Danguy, Catherine A. Hamilton, Karen M. Stewart, Marie-Isabel Aguilar, and A. Ian Smith 10 A Convenient Method for the Synthesis of Cyclic Peptide Libraries Gregory T. Bourne, Jonathon L. Nielson, Justin F. Coughlan, Paul Darwen, Marc R. Campitelli, Douglas A. Horton, Andreas Ruhmann, Steve G. Love, Tran T. Tran, and Mark L. Smythe 11 High Throughput Peptide Synthesis Michal Lebl and John Hachmann 12 Backbone Amide Linker Strategies for the Solid-Phase Synthesis of C-Terminal Modified Peptides Jordi Alsina, Steven A. Kates, George Barany, and Fernando Albericio 13 Synthesis of Peptide Bioconjugates Ferenc Hudecz Part III: Practical Guides 14 Protein Identification by Mass Spectrometric Analyses of Peptides Ashley Martin 15 Manual Solid Phase Synthesis of Glutathione Analogues. A Laboratory-Based Short Course Ursel Soomets, Mihkel Zilmer, and Ulo Langel

Customer Reviews

Handbook / Manual
Series: Methods in Molecular Biology Volume: 298
By: John Howl
Publisher: Humana Press
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