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Plant Cell Culture Protocols: Methods and Protocols

Handbook / Manual

Series: Methods in Molecular Biology Volume: 877

By: Víctor M Loyola-Vargas(Editor), Neftalí Ochoa-Alejo(Editor)

444 pages, 18 colour & 28 b/w illustrations, 27 tables

Humana Press

Hardback | May 2012 | Edition: 3 | #205725 | ISBN-13: 9781617798177
Availability: Usually dispatched within 1 week Details
NHBS Price: £119.99 $153/€134 approx

About this book

Cell culture methodologies have become standard procedures in most plant laboratories. Currently, facilities for in vitro cell cultures are found in practically every plant biology laboratory, serving different purposes since tissue culture has turned into a basic asset for modern biotechnology, from the fundamental biochemical aspects to the massive propagation of selected individuals. Plant Cell Culture Protocols is divided into five convenient sections that cover topics from general methodologies, such as culture induction, growth and viability evaluation, statistical analysis and contamination control, to highly specialized techniques, such as clonal propagation, haploid production, somatic embryogenesis, organelle transformation. Plant Cell Culture Protocols concludes with a section on the laborious process of measuring the epigenetics changes in tissue cultures.

Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Plant Cell Culture Protocols seeks to serve both professionals and novices with its guide to the most common and applicable techniques and methods for plant tissue and cell culture.


1. An Introduction to Plant Cell Culture Victor M. Loyola-Vargas and Neftali Ochoa-Alejo
2. History of Plant Tissue Culture Trevor Thorpe
3. Callus, Suspension Culture and Hairy Roots. Induction, Maintenance, and Characterization Rosa M. Galaz-Avalos, Sagrario Aguilar-Diaz, Pedro A. Xool-Gonzalez, Silvia M. Huchin-May and Victor M. Loyola-Vargas
4. Growth Measurements. Estimation of Cell Division and Cell Expansion Gregorio Godoy-Hernandez and Felipe A. Vazquez Flota
5. Measurement of Cell Viability Lizbeth A. Castro-Concha, Rosa Maria Escobedo, and Maria de Lourdes Miranda-Ham
6. Pathogen and Biological Contamination Management in Plant Tissue Culture: Phyto-Pathogens, Vitro-Pathogens, and Vitro-Pests Alan C. Cassells
7. Cryopreservation of Embryogenic Cell Suspensions by Encapsulation-Vitrification and Encapsulation-Dehydration Zhenfang Yin, Long Chen, Bing Zhao, Yongxing Zhu, and Qiaochun Wang
8. The Study of in vitro Development in Plants: General Approaches and Photography Edward C. Yeung
9. Use of Statistics in Plant Biotechnology Michael E. Compton
10. Tissue Culture Methods for the Clonal Propagation and Genetic Improvement of Spanish Red Cedar (Cedrela odorata) Yuri Pena-Rodriguez, Juan Juarez Gomez, Jose Antonio Gonzalez, and Manuel L. Robert
11. Micropropagation of Banana Yildiz Aka Kacar and Ben Faber
12. Liquid in vitro Culture for the Propagation of Arundo donax Miguel Angel Herrera-Alamillo and Manuel L. Robert
13. Production of Haploids and Doubled Haploids in Maize Vanessa Prigge and Albrecht E. Melchinger
14. Maize Somatic Embryogenesis: Recent Features to Improve Plant Regeneration Veronica Garrocho-Villegas, Maria Teresa de Jesus-Olivera, and Estela Sanchez Quintanar
15. Improved Shoot Regeneration from Root Explants Using an Abscisic Acid Containing Medium Subramanian Paulraj and Edward C. Yeung
16. Cryopreservation of Shoot Tips and Meristems: An Overview of Contemporary Methodologies Erica E. Benson and Keith Harding
17. Anther Culture of Chili Pepper (Capsicum spp.) Neftali Ochoa-Alejo
18. Production of Inter-Specific Hybrids in Ornamental Plants Juntaro Kato and Masahiro Mii
19. Plant Tissue Culture of Fast-Growing Trees for Phytoremediation Research Jose Luis Couselo, Elena Corredoira, Ana M. Vieitez, and Antonio Ballester
20. Removing Heavy Metals by in vitro Cultures Maria del Socorro Santos-Diaz and Maria del Carmen Barron-Cruz
21. Establishment of a Sanguinarine Producing Cell Suspension Culture of Argemone Mexicana L. (Papaveraceae). Induction of Alkaloid Accumulation Felipe A. Vazquez-Flota, Miriam Monforte-Gonzalez, Cecilia Guizar-Gonzalez, Jorge Rubio-Pina, and Karen Trujillo-Villanueva
22. Epigenetics, the Role of DNA Methylation on Tree Development Marcos Viejo Somoano, Maria E. Santamaria, Jose L. Rodriguez, Luis Valledor, Monica Meijon, Marta Perez, Jesus Pascual, Rodrigo Hasbun, Mario Fernandez Fraga, Maria Berdasco, Peter E. Toorop, Maria J. Canal, and Roberto Rodriguez Fernandez
23. The Potential Roles of microRNAs in Molecular Breeding Qing Liu and Yue-Qin Chen
24. Determination of Histone Methylation in Mono- and Dicotyledons Plants Geovanny I. Nic-Can and Clelia De la Pena
25. Basic Procedures for Epigenetic Analysis in Plant Cell and Tissue Culture Jose L. Rodriguez, Jesus Pascual, Marcos Viejo Somoano, Luis Valledor, Monica Meijon, Rodrigo Hasbun, Norma Yague Yrei, Maria E. Santamaria, Marta Perez, Mario Fernadez Fraga, Maria Berdasco, Roberto Rodriguez Fernandez, and Maria J. Canal
26. Plant Tissue Culture and Molecular Markers Maria Tamayo-Ordonez, Javier Huijara-Vasconselos, Adriana Quiroz-Moreno, Matilde Ortiz-Garcia, and Felipe Sanchez-Teyer
27. Biolistic- and Agrobacterium-Mediated Transformation Protocols for Wheat Cecilia Tamaz-Nyitrai, Huw D. Jones, and Laszlo Tamas
28. Improved Genetic Transformation of Cork Oak (Quercus suber L.) Ruben Alvarez-Fernandez and Ricardo-Javier Ordas
29. Organelle Transformation Anjanabha Bhattacharya, Anish Kumar, Nirali Desai, and Seema Parikh
30. Appendix A - The Components of the Culture Media Victor M. Loyola-Vargas
31. Appendix B - Plant Biotechnology and Tissue Culture Resources in the Internet Victor M. Loyola-Vargas

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