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A comprehensive state-of-the-art collection of the most frequently used techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. Two new appendices explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, and list more than 100 very useful internet sites.
Introduction. An Introduction to Plant Cell Culture: Pointers to Success R. D. Hall. Cell Culture and Plant Regeneration. Callus Initiation Maintenance and Shoot Induction in Rice N. W. Blackhall J. P. Jotham K. Azakanandam J. B. Power K. C. Lowe E. C. Cocking and M. R. Davey. Callus Initiation Maintenance and Shoot Induction in Potato:Monitoring of Spontaneous Genetic Variability In Vitro and In Vivo R. F. Curry and A. C. Cassells. Somatic Embryogenesis in Barley Suspension Cultures M. Kihara H. Funatsuki K. Ito and P. A. Lazzeri. Somatic Embryogenesis in Picea Suspension Cultures U. Egertsdotter. Specialized Techniques. Direct Cyclic Somatic Embryogenesis in Cassava for Mass Production Purposes K. J. J. M. Raemakers E. Jacobsen and R. G. F. Visser. Immature Inflorescence Culture of Cereals S. Rasco-Gaunt and P. Barcelo. Cryopreservation of Rice Tissue Cultures E. E. Benson and P. T. Lynch. Non-Cryogenic Long-Term Germplasm Storage of Potato A. Golmirzaie and J. Toledo. Plant Propagation In Vitro. Microprogation of Strawberry via Axillary Shoot Proliferation P. Boxus. Meristem-Tip Culture for Propagation and Virus Elimination B. W. W. Grout. Specialized Techniques. Clonal Propagation of Orchids B. Tisserat and D. Jones. Clonal Propagation of Cacti J. Gratton and M. F. Fay. Microproragation of Flower Bulbs: Lily and Narcissus M. M. Langens-Gerrits and G-J . M. de Klerk. Clonal Propagation of Conifers I. S. Harry and T. A. Thorpe. The Culture of Ferns M. V. Ford and M. F. Fay. Applications for Plant Protoplasts. Protoplast Isolation Culture and Plant Regeneration from Passiflora Leaves P. Anthony W. Otoni J. B. Power K. C. Lowe and M. R. Davey. Isolation Culture and Plant Regeneration from Suspension-Derived Protoplasts of Lolium M. Folling and A. Olesen. Protoplast Fusion for Symmetric Somatic Hybrid Production in Brassicaceae J. Fahleson and K. Glimelius. Production of Cybrids in Rapeseed (Brassica napus) S. Yarrow. Specialized Techniques. Microprotoplast Isolation and the Production of Monosomic Addition Lines K. S. Ramulu. Guard Cell Protoplasts: Isolation Culture and Regeneration of Plants G. Boorse and G. Tallman. In Vitro Fertilization with Isolated Single Gametes E. Kranz. Protocols for Genomic Manipulation. Anther and Microspore Culture in Barley H. Lorz and A. J"hne-G"rtner. Microspore Embryogenesis and In Vitro Pollen Maturation for the Production of (Double) Haploids in Tobacco E. Heberle-Bors. Embryo Rescue Following Wide Crosses H. C. Sharma. Specialized Techniques. Mutagenesis and the Selection of Resistant Mutants P. J. Dix. The Generation of Plastid Mutants In Vitro P. J. Dix. Protocols for the Introduction of Specific Genes. Agrobacterium-Mediated Transformation of Petunia Leaf Discs I. M. van der Meer. Transformation of Rice Via PEG-Mediated DNA Uptake into Protoplasts K. Datta and S. K. Datta. Transformation of Wheat Via Particle Bombardment I. K. Vasil and V. Vasil. Plant Transformation via Protoplast Electroporation G. W. Bates. Specialized Techniques. Transformation of Maize via Tissue Electroporation K. D'Halluin E. Bonne M. Bossut and R. Le Page. Transformation of Maize Using Silicon Carbide Whiskers J. M. Dunwell. Suspension Culture Initiation and the Accumulation of Metabolites. Directing Anthraquinone Accumulation via Manipulation of Morinda Suspension Cultures M. J. M. Hagendoorn D. C. L. Jamar and L. H. W. van der Plas. Alkaloid Accumulation in Catharanthus roseus Suspension Cultures A. H. Scragg. Betalains: Their Accumulation and Release In Vitro C.S. Hunter and N. J. Kilby. Appendix. Index.
... a valuable update on the technology, with emphasis on methods for micropropagation and gene transfer...the editor has assembled an excellent range of expert authors and the volume is well balanced and up-to-date...thoroughly recommended. - Plant Growth Regulation Protocols are presented for many agronomically important plants including the culture and regeneration of rice, potato, barley, casava, spruce, Pasiflora, Lolium, and various species of Brassica. The organization of all of the chapters is consistent including an introduction, a materials section divided into appropriate subsections, a similarly subdivided methods section, a notes section that contains important observations to make or precautions to take, and a final reference section...the techniques are well written and include specific examples and step-by-step methods with appropriate references...will make an excellent addition to the library of researchers who wish to extend their collection of basic plant cell culture techniques. - Quarterly Review of Biology The combination of many years of experience with new experimental entries is often very successful and of high quality represents one of the highest quality manuals on this topic published with the last ten years it will assure the relevance of an important scientific discipline, plant biotechnology, in today's world. - Biologia Plantarum The book brings together a series of well written, step-by-step protocols on the most frequently used techniques in plant cell and tissue culture, covering various aspects of culture initiation, maintenance, long-term storage and manipulation. In addition to protocols on widely applied methods, each part includes representative protocols for more specialized techniques. All the chapters are well structured, profusely illustrated with original photographs and line drawings, and contain detailed descriptions of materials, culture media and comprehensively described experimental procedures. The reader will certainly appreciate the precautionary notes and additional hints on each technique and each protocol provided at the end of each chapter. - Elsevier This book presents a comprehensive collection of applicable techniques for plant cell and tissue culture. The methods cover culture initiation, maintenance, manipulation, application and long-term storage, with emphasis on techniques for genetic modification and micropropagation. - Lavoisier Librairie
