This completely revised and updated edition takes advantage of the many new developments and applications that have occurred in PRINS and in situ PCR technology, including a new fast multicolor PRINS method for identifying human chromosomes, PRINS protocols for in situ detection of unique sequences and point mutation analysis, a new dideoxy PRINS procedure, and in situ PCR and PRINS protocols for plants. The authors describe in detail various applications of PRINS in human (detection of gene deletions in cancer detection of fetal cells in maternal blood, and assessment of aneuploidy in brain tissues and embryos) as well as plant cells. Readily reproducible in situ PCR techniques are also presented for the detection of cytomegalovirus, and for use in combination with microdissection. In situ RT-PCR techniques are also given for use in plant or cancer investigation.
...[a] splendid little book which covers the field quite well...Chapetr 16, which is a general review of in situ PCR written by Aidan Long and Paul Komminoth...is by far the best review of the approach...yet seen and should be mandatory reading for anyone intending to try in situ PCR. - FEBS Letters useful to all those increasingly applying such techniques to in situ analysis this book contains clear practical instructions that are easy to follow, and is well illustrated. It is essential for any laboratory contemplating exploiting this powerful technique. - SGM Quarterly
Oligonucleotide PRINS DNA Synthesis John R. Gosden and Diane Lawson. Chromosome-Specific PRINS Jean-Paul Charlieu and Franck Pellestor. Bright-Field Microscopic Detection of Oligonucleotide PRINS-Labeled DNA in Chromosome Preparations Ernst J. M. Speel Daine Lawson Frans C. S. Ramaekers John R. Gosden and Anton H. N. Hopman. Analysis of Sperm Aneuploidy by PRINS Franck Pellestor and Jean-Paul Charlieu. PRINS DNA Synthesis on Frozen Tissue Sections Ernst J. M. Speel Diane Lawson Frans C. S. Ramaekers John R. Gosden and Anton H. N. Hopman. Multiple Sequential Oligonucleotide Primed In Situ DNA Synthesis (MULTI-PRINS) John R. Gosden and Diane Lawson. Oligonucleotide PRINS DNA Synthesis on Extended Chromatin Preparations Yoshiro Shibasaki and John R. Gosden. Combined Immunocytochemistry and PRINS DNA Synthesis for Simultaneous Detection of Phenotypic and Genomic Parameters in Cells Ernst J. M. Speel Diane Lawson Frans C. S. Ramaekers John R. Gosden and Anton H. N. Hopman. Chromosomal PRINS DNA Labeling Combined with Indirect Immunocytochemistry Arthur R. Mitchell. Direct In Situ Single-Copy (DISC)-PCR Deryl Troyer Hongyan Xie and Jie Hu. In Situ PCR to Cells and to Wax Sections: An Alternative to In Situ Hybridization for the Detection of Low Copies of Human Papillomavirus and Cytomegalovirus DNA Christiane Mougin Jean Marc Didier Dominique Bettinger Laurence Madoz Sylviane Coumes-Marquet and Michel Lab. Reverse Transcriptase PCR In Situ on Cryopreserved Tissue Sections Fred J. Hendler Alice Shum-Siu Briana W. Heniford Mohammed El Mekki Ahmed and V. J. Patel. Flow Cytometry Analysis of an In Situ PCR for the Detection of Human Immunodeficiency Virus Type-1 (HIV-1) Proviral DNA David E. Gibellini Maria Carla Re Giuliano Furlini and Michele La Placa. In Situ PCR Amplification of Intracellular mRNA Raymond H. Chen and Susan V. Fuggle. Localized In Situ Amplification (LISA): PCR Amplification of Nucleic Acid Sequences in Tissue Sections Gregory J. Tsongalis. In Situ PCR: An Overview Aidan A. Long and Paul Komminoth. Index.
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