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This volume is an up date of the previous edition, Protein Targeting Protocols, placing the emphasis on protein targeting to cellular compartments in both prokaryotic and eukaryotic systems. State-of-the-art information is provided on the fast moving fields of import machinery of mitochondria and plastids, as well as a detailed protocol described to follow the movement of protein complexes in bacterial membranes using fluorescent recovery after photobleaching.
Surface Plasmon Resonance: Measuring Protein Interactions in Real Time, G. Panayotou. Microcalorimetry of Protein Protein Interactions, A. Cooper. Partitioning of Proteins in Triton X-114, J. G. Pryde. Co-immunoprecipitation: Identification of Interacting Proteins, N. G. Anderson. Overlay, Ligand Blotting, and Band-Shift Techniques to Study Kinase Anchoring, Z. E. Hausken, V. M. Coghlan, and J. D. Scott. Size-Exclusion Chromatography: Identification of Interacting Proteins, J. Beattie. Overlay and Bead Assay: Determination of Calcium Channel Subunit Interaction Domains, V. E. S. Scott, C. A. Gurnett, and K. P. Campbell. Use of GST-Fusion and Related Constructs for the Identification of Interacting Proteins, M. Harris. Molecular Genetic Approaches I: Two Hybrid Systems, G. B. Bolger. Molecular Genetic Approaches II: Expression Interaction Cloning, M. Csukai and D. Mochly-Rosen. Molecular Genetic Approaches III: Determination of Protein Sequence Motifs Involved in Protein Targeting by Use of Coupled Transcription Translation Systems, G. Scotland and M. D. Houslay. Molecular Genetic Approaches IV: Recombinant Expression of Wild-Type and Acylation-Resistant G-Protein a-Subunits Using Transient Transfection Systems, M. A. Grassie and G. Milligan. Use of Synthetic Peptides in the Dissection of Protein-Targeting Interactions, J. D. Scott and M. C. Faux. SH2 and SH3 Domains: Unraveling Signaling Networks with Peptide Antagonists, R. Stein. PH Domain of Serine Threonine Protein Kinase B (RAC-PKB): Expression and Binding Assay for Phosphoinositides and Inositol Phosphates, M. Frech and B. A. Hemmings. Membrane Targeting via Protein N-Myristoylation, R. A. J. McIlhinney. Membrane Targeting via Protein Palmitoylation, M. Veit and M. F. G. Schmidt. Agonist-Mediated Turnover of G-Protein a-Subunit Palmitoyl Groups: Role in Membrane Insertion, M. A. Grassie and G. Milligan. Membrane Targeting via Protein Prenylation, J. H. Overmeyer, R. A. Erdman, and W. A. Maltese. Nuclear Protein Import in a Permeabilized Cell Assay, F. Melchior. Clathrin-Coated Vesicles and Targeting: Preparation of Adaptor Proteins, A. Pauloin. Analysis of the Sorting of Secretory Proteins to the Regulated Secretory Pathway: A Subcellular Fractionation Approach, E. Chanat, A. S. Dittie, and S. A. Tooze. Index.