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Quantum Dots: Biological Applications is the first book published on the subject, and it emphasizes a variety of methods that were developed using commercially available materials. Each of the protocols in this authoritative, cutting-edge text have been prepared and written by the developers of novel applications for quantum dots, many of whom have published the initial reports on the subject in major journals. This comprehensive volume outlines nearly all currently developed applications of quantum dots in precise detail. The protocols will be of tremendous interest to experimental biologists, as they examine current and potential applications of quantum dot labeling in the field. Cutting-edge examples of potential biological applications for quantum dots include cancer detection, live cell tracking, and pathology. This volume provides an excellent reference for protocols that can be utilized repeatedly to yield reliable performance from quantum dot-enabled applications.
Table of Contents; Preface; Contributors; Part 1 - Imaging of fixed cells and tissues; 1. Immunofluorescent Labeling of Proteins in Cultured Cells with Quantum Dot Secondary Antibody Conjugates; Richard L. Ornberg and Hongjian Liu; 2. Immunohistochemical Detection with Quantum Dots; Rizwan S. Akhtar, Cecelia B. Latham, Dario Siniscalco, Carlo Fuccio and Kevin A. Roth; 3. Quantum Dots for Multicolor Clinical Pathology and Multispectral Imaging; Johbu Itoh and Robert Yoshiyuki Osamura; 4. Light and Electron Microscopic Localization of Multiple Proteins using Quantum dots; Thomas J. Deerinck, Ben N. G. Giepmans, Benjamin L. Smarr, Maryann E. Martone and Mark H. Ellisman; 5. Human Metaphase Chromosome FISH using Quantum Dot Conjugates; Joan H.M. Knoll; Part 2 - Imaging of live cells; 6. Biotin-Ligand Complexes with Streptavidin Quantum Dots for in vivo Cell Labeling of Membrane Receptors; Diane S. Lidke, Peter Nagy, Thomas M. Jovin and Donna J. Arndt-Jovin; 7. Single Quantum Dot Tracking of Membrane Receptors; Cedric Bouzigues, Sabine Levi, Antoine Triller and Maxime Dahan; 8. Optical Monitoring of Single Cells Using Quantum Dots; Jyoti K. Jaiswal and Sanford M. Simon; 9. Peptide Mediated Intracellular Delivery of Quantum Dots; B. Christoffer Lagerholm; 10. High Content Analysis in Multiple Cell Lines Using Quantum Dots; Paul G. Wylie; 11. Measuring Cell Motility Using Quantum Dot Probes; Weiwei Gu, Teresa Pellegrino, Wolfgang J. Parak, Rosanne Boudreau, Mark A. Le Gros, A. Paul Alivisatos and Carolyn A. Larabell; Part 3 - Imaging of live animals; 12. Quantum Dots for In-vivo Molecular and Cellular Imaging; Xiaohu Gao, Leland WK Chung and Shuming Nie; 13. Sentinel Lymph Node Mapping with Type II Quantum Dots; John V. Frangioni, Sang-Wook Kim, Shunsuke Ohnishi, Sungjee Kim and Moungi Bawendi; 14. Macrophage-mediated Co-localization of Quantum Dots in Experimental Glioma; Osman Muhammad, Alexandra Popescu and Steven A. Toms; Part 4 - Flow cytometry applications. 15. Applications of Quantum Dots to Multicolor Flow Cytometry; Pratip K. Chattopadhyay and Mario Roederer; 16. Quantum Dots in Flow Cytometry; Barnaby Abrams and Tim Dubrovsky; Part 5 - Biochemical applications; 17. Luminescent Biocompatible Quantum Dots: A tool for immunosorbent assay design; Ellen R. Goldman, H. Tetsuo Uyeda, Andrew Hayhurst, and Hedi Mattoussi; 18. Fluorescence-based Analysis of Cellular Protein Lysate Arrays Using Quantum Dots; David H. Geho, J. Keith Killian, Animesh Nandi, Johanne Pastor, Prem Gurnani and Kevin P. Rosenblatt.; 19. Application of Quantum Dots to Multi-Color Microarray Experiments: Four-Color Genotyping; George Karlin-Neumann, Marina Sedova, Mat Falkowski, Zhiyong Wang, Steven Lin and Maneesh Jain; Index.
From the reviews: "...provides an excellent reference for protocols that can be utlized repeatedly to yield reliable performance from quantum dot-enabled applications." -Anticancer Research 27, May 2007 "Bruschez and Holtz have compiled an extremely useful set of applications and protocols in Quantum Dots, Applications in Biology. The protocols, written by the many leaders in QD imaging, cover a wide range of applications and provide detailed methodology for each application supported by excellent illustrations. This book certainly belongs on the shelf of anyone interested in biological imaging. ! in summary, this illustrative compilation of well-written protocols on QD is a must have for both the expert and novice interested in biological imaging." (Neel Ghosh, ChemBioChem, Issue 9, 2008)