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The past decade has seen the emergence of plants as a viable alternative to the current production systems for therapeutic proteins. In Recombinant Proteins from Plants, expert researchers explore plants and their potential for the production of increasingly safe, high quality and biologically active complex recombinant pharmaceutical proteins. Chapters present several production platforms, focusing on the largest and most important group of biotechnological products in clinical trials: antibodies and their derived fragments with acknowledged potential for immunotherapy in humans. Due to the shortage of step by step protocols that can be used easily by beginners, this volume aims to fill the void with several chapters of detailed instructions for using the main plant expression systems.
Composed in the highly successful "Methods in Molecular Biologya" series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Recombinant Proteins from Plants serves as an ideal guide for those new and seasoned plant scientists, pharmaceutical scientists, and molecular and cellular biologists interested in molecular pharming.
1. From Neanderthal to Nanobiotech: from plant potions to pharming with plant factories Christophe Sourrouille, Brian Marshall, David Lienard and Loic Faye 2. Cowpea Mosaic Virus-based systems for the expression of antigens and antibodies in plants. Frank Sainsbury, Li Liu and George P. Lomonossoff 3. Transient expression of antibodies in plants using syringe agroinfiltration Marc-Andre D'Aoust, Pierre-Olivier Lavoie, Julie Belles-Isles, Nicole Bechtold, Michele Martel and Louis-P. Vezina 4. Rapid system for evaluating bioproduction capacity of complex pharmaceutical proteins in plants Giuliana Medrano, Michael J. Reidy, Jianyun Liu, Jorge Ayala, Maureen C. Dolan and Carole L. Cramer 5. Production and localization of recombinant pharmaceuticals in transgenic seeds Thomas Rademacher, Elsa Arcalis and Eva Stoger 6. Production of antibody fragments in Arabidopsis seeds Bart Van Droogenbroeck, Kirsten De Wilde and Ann Depicker 7. Production of plantibodies in Nicotiana plants Marta Ayala, Jorge Gavilondo, Meilyn Rodriguez, Alejandro Fuentes, Gil Enriquez, Lincidio Perez, Jose Cremata, and Merardo Pujol. 8. Physcomitrella patens: a non-vascular plant for recombinant protein production David Lienard and Fabien Nogue 9. Production of recombinant proteins in suspension-cultured plant cells Carole Plasson, Remy Michel, David Lienard, Claude Saint-Jore-Dupas, Christophe Sourrouille, Ghislaine Grenier de March and Veronique Gomord 10. Chloroplast-derived vaccine antigens and biopharmaceuticals: Protocols for expression, purification or oral delivery and functional evaluation N. Dolendro Singh, Yi Ding and Henry Daniell 11. Protein body induction: a new tool to produce and recover recombinant proteins in plants Margarita Torrent, Imma Llop and M.Dolors Ludevid 12. A case study for plant-made pharmaceuticals comparing different plant expression and production systems Guy Vancanneyt, Manuel Dubald, Werner Schroder, Jorg Peters and Johan Botterman 13. Glycosylation of antibody therapeutics: optimisation for purpose Roy Jefferis 14. N-Glycosylation of Plant Recombinant Pharmaceuticals Muriel Bardor, Gleysin Cabrera, Johannes Stadlmann, Patrice Lerouge, Jose A. Cremata, Veronique Gomord and Anne-Catherine Fitchette 15. Companion protease inhibitors to protect recombinant proteins in transgenic plant extracts Meriem Benchabane, Daniel Rivard, Cecile Girard, and Dominique Michaud 16. Strategies for improving vaccine antigens expression in transgenic plants. Fusion to carrier sequences Jose M. Escribano and Daniel M. Perez-Filgueira 17. Immunomodulation of plant function by in vitro selected single-chain Fv intrabodies Manfred Gahrtz and Udo Conrad 18. On-chip detection of low-molecular-weight recombinant proteins in plant crude extracts by SELDI-TOF MS Amine M. Badri, Karine Coenen, Louis-Philippe Vaillancourt, Charles Goulet, and Dominique Michaud 19. Assessing the Risk of Undesirable Immunogenicity/Allergenicity of Plant-Derived Therapeutic Proteins Paul D. Chamberlain 20. Biosafety, Risk Assessment and Regulation of Plant-made Pharmaceuticals Penelope A.C.Sparrow and Richard M.Twyman
From the reviews: "This book presents current methods for the expression of those pharmaceutical proteins in a variety of plant systems. ! Each one of its twenty chapters offers objective, clearly written texts including a summary ! and some important notes followed by the references. ! 'Recombinant proteins from plants' is surely a useful tool and a practical and rapid summarized data source with easy procedures on plant molecular pharming for all scientists and related professionals working in this growing field." (Dominique C. H. Fischer, Brazilian Journal of Pharmaceutical Sciences, 2009)