Serial Analysis of Gene Expression (SAGE) Digital Gene Expression Profiling

About this book

Visual Prosthesis and Ophthalmic Devices facilitates the analysis and comparison of data from SAGE experiments in a correct and efficient way, as well as enables users inexperienced with SAGE and having only limited experience in standard molecular biology techniques to conduct SAGE experiments. The volume is comprised of two parts. The first is concerned with experimental procedures of SAGE and related methods, while the second focuses on the correct extraction and filtering of tags, analysis of digtag populations, and how to carry out statistically correct comparisons of gene expression profiles.

Contents

11. Duplicate Ditag analysis in LongSAGE; Jeppe Emmersen.; 12. Statistical Comparison of Two or More SAGE Libraries: One Tag at the Time.; Gerben J. Schaaf, Fred van Ruissen, Antoine van Kampen, Marcel Kool and Jan M. Ruijter.; 13. Scaling of Gene Expression Data Allowing the Comparison of Different Gene Expression Platforms.; Fred van Ruissen, Gerben J. Schaaf, Marcel Kool, Frank Baas and Jan M. Ruijter.; 14. Clustering Analysis of SAGE Transcription Profiles Using a Poisson Approach.; Haiyan Huang, Li Cai and Wing H. Wong.; 15. Identifying Non-specific SAGE tags by Context of Gene Expression.; Xijin Ge and San Ming Wang.

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