Target Discovery and Validation: Reviews and Protocols, Volumes 1 and 2 review the most progressive and current methods for drug target discovery and validation. These volumes explore how recent improvement in understanding the molecular mechanisms of human pathology is impacting drug target discovery in the laboratory and in real therapeutics, specifically for cancers and autoimmune disorders.
Volume 1 focuses on novel and innovative techniques, and presents the most up-to-date protocols available for maximizing the likelihood of achieving target-selective inhibition in vivo while minimizing side effects. The profound impact of genomics, proteomics and bioinformatics on target discovery is explored, and specific attention is given to the role of transgenic and knockout animals in functional genomics and target validation. Cancer researchers will find tremendous value in the molecular classification of breast cancers and the review of protocols for tumor antigens and cancer vaccines. The methods and protocols collected here, all reviewed by leading scientists and clinicians, present the practical details necessary for translating the enormous discovery potential of the genome into real therapeutic products. Volume 2 collects all the practical details required for efficient translation of discovered targets into real pharmaceutical drugs. Specific targets in cancers and autoimmunity are described and the potential of using siRNA's, antisense oligonucleotides and RNA aptimers in patients is reviewed. This volume explores the tremendous impact of the application of genotyping and gene expression profiling on the future of healthcare, and presents cutting-edge protocols to aid in bringing agents against specific targets closer to application in the clinic. Collectively, these volumes provide a thorough review of the most cutting-edge methods available for each step in drug target identification, validation, and clinical application. For researchers, an understanding of available methods aids in the creation of innovative experiments in the laboratory, and the successful translation of target discovery to real therapeutics.
Target Discovery and Validation: Reviews and Protocols Part I: Emerging strategies for targets and biomarker discovery 1. Main approaches to target discovery and validation Mouldy Sioud 2. Bioinformatics approaches to cancer gene discovery Ramaswamy Narayanan 3. Analysis of gene networks for drug target discovery and validation Seiya Imoto, Yoshinori Tamada, Christopher J. Savoie, and Satoru Miyano 4. Target discovery and validation in pancreatic cancer Robert M. Beaty, Mads Gronborg, Jonathan R. Pollack, and Anirban Maitra 5. Molecular classification of breast tumors: Clinical impact on diagnosis, prognosis, and treatment Therese Sorlie 6. Discovery of differentially expressed genes: Technical considerations A ystein Rosok and Mouldy Sioud 7. Genome-wide screening using siRNA-expression libraries Sahohime Matsumoto, Makoto Miyagishi, Ryozo Nagai, and Kazunari Taira 8. Hammerhead ribozyme-based target discovery Masayuki Sano and Kazunari Taira 9. Production of siRNA and cDNA transfected cell arrays on non-coated chambered coverglass for high content screening microscopy in living cells Holger Erfle and Rainer Pepperkok 10. Transgenic animal models in biomedical research Louise-Marie Houdebine 11. Keratin transgenic and knockout mice: Functional analysis and validation of disease-causing mutations Preethi Vijayaraj, Goran Sohl, and Thomas M. Magin 12. The HUVEC/Matrigel assay - an in vivo assay of human angiogenesis suitable for drug validation Dag K. Skovseth, Axel M. Kuchler, and Guttorm Haraldsen 13. A murine model for studying hematopoiesis and immunity in heart failure Per Ole Iversen and Dag R. Sorensen 14. An overview of the immune system and technical advances in tumor antigen discovery Mouldy Sioud 15. Potential target antigens for immunotherapy identified by serological expression cloning (SEREX) Dirk Jager 16. Identification of tumor antigens using proteomics Francoise Le Naour 17. Protein arrays: a versatile toolbox for target identification and monitoring of patient immune responses Lina Cekaite, Eivind Hovig and Mouldy Sioud 18. Considerations for target validation and industrial approaches Carlos R. Plata-Salaman, and Sergey E. Ilyin
From the reviews: "Volume 1 focuses on novel and innovative techniques, and presents the most up-to-date protocols available for maximizing the likelihood of achieving target-selective inhibition in vivo while minimizing side effects. ! Cancer researchers will find tremendous value in the molecular classification of breast cancers and the review of protocols for tumor antigens and cancer vaccines. ! provide a thorough review of the most cutting-edge methods available for each step in drug target identification, validation, and clinical application." (Anticancer Research, Vol. 27, May, 2007) "Review current methods for drug target discovery and validation. ! Volume 1 is focused on drug target and biomarker discovery. ! the chapters are generally well written and clear, and provide extensive references to the current literature. ! Overall ! provide useful overviews that would provide entry into this field. Novice and more experienced researchers ! would find useful information ! ." (Jonathan B. Chaires, ChemMedChem, Issue 3, 2008)