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Therapeutic Proteins: Methods and Protocols

Handbook / Manual

Series: Methods in Molecular Biology

By: C Mark Smales and David C James

Humana Press

Hardback | Dec 2005 | #163700 | ISBN: 1588293904
Availability: Usually dispatched within 1-2 weeks Details
NHBS Price: £159.99 $210/€182 approx

About this book

World-class researchers share their innovative and proven techniques for the production of therapeutic proteins downstream of the discovery stage. The methods use a variety of sources for producing therapeutic proteins, including bacterial and yeast expression systems, and insect and mammalian cells, and then cover the purification of the resulting protein using both state-of-the-art and traditional methods, such as those sourced from plasma. Additional chapters offer methods for the characterization of therapeutic proteins throughout the production process, along with methods and strategies for viral inactivation and protein formulation. The protocols follow the successful Methods in Molecular BiologyT series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.


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