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Academic & Professional Books  Organismal to Molecular Biology  Biochemistry & Molecular Biology

Vaccine Protocols

Handbook / Manual
By: Andrew P Robinson(Editor), Michael J Hudson(Editor), Martin P Cranage(Editor)
428 pages, b/w illustrations
Publisher: Humana Press
Vaccine Protocols
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  • Vaccine Protocols ISBN: 9781617373824 Edition: 2 Paperback Oct 2010 Usually dispatched within 1-2 weeks
    £138.00
    #208477
  • Vaccine Protocols ISBN: 9781588291400 Edition: 2 Hardback Aug 2003 Usually dispatched within 1-2 weeks
    £139.99
    #208476
Selected version: £138.00
About this book Contents Customer reviews Related titles

About this book

This extensively revised, updated, and expanded second edition of a classic laboratory manual applies new understanding of molecular mechanisms to the rational design and development of vaccines. Here the working researcher will find readily reproducible techniques for the development and production of live attenuated vaccines, and vaccines based on inactivated toxins, peptides, and polysaccharide conjugates. New to this edition are cutting-edge chapters on two different viral vectors (Vaccinia and Semliki Forest), lactic acid bacteria for delivery of foreign antigens, incorporation of immunomodulators into plasmid DNA vaccines, construction of tetramer complexes to detect CMI responses, assessment of functional antibody responses, and the use of complete genome sequences for vaccine design. There are also chapters describing more general techniques for vaccine formulation, delivery, and assessment of immune responses, and review discussions of scale-up to manufacture, vaccine quality assurance, and clinical trials.

Contents

Overview of Vaccines Gordon Ada Temperature-Sensitive Mutant Vaccines Craig R. Pringle Live Viral Vectors: Construction of a Replication-Deficient Recombinant Adenovirus Anthony R. Fooks Live Viral Vectors: Vaccinia Virus Caroline Staib and Gerd Sutter Live Viral Vectors: Semliki Forest Virus Gunilla B. Karlsson and Peter J. Liljestrom Development of Attenuated Salmonella Strains That Express Heterologous Antigens Frances Bowe, Derek J. Pickard, Richard J. Anderson, Patricia Londono-Arcila, and Gordon Dougan Expression and Delivery of Heterologous Antigens Using Lactic Acid Bacteria Mark A. Reuter, Sean Hanniffy, and Jerry M. Wells Synthetic Peptides Michael J. Francis Genetic Detoxification of Bacterial Toxins Mariagrazia Pizza, Maria Rita Fontana, Vincenzo Scarlato, and Rino Rappuoli Preparation of Polysaccharide-Conjugate Vaccines Carla C. A. M. Peeters, Patrick R. Lagerman, Odo de Weers, Lukas A. Oomen, Peter Hoogerhout, Michel Beurret, Karen M. Reddin, and Jan T. Poolman Adjuvant Formulations for Experimental Vaccines Duncan E. S. Stewart-Tull Incorporation of Immunomodulators into Plasmid DNA Vaccines Linda S. Klavinskis, Philip Hobson, and Andrew Woods Microencapsulation of Vaccine Antigens David H. Jones Lyophilization of Vaccines: Current Trends Gerald D. J. Adams Stimulation of Mucosal Immunity David J. M. Lewis and Christopher M. M. Hayward Induction and Detection of T-Cell Responses Kingston H. G. Mills Construction of MHC Class I-Peptide Tetrameric Complexes for Analysis of T-Cell-Mediated Immune Responses Rachel V. Samuel and Tomas Hanke Assessment of Functional Antibody Responses Ray Borrow and Paul Balmer The Use of Complete Genome Sequences in Vaccine Design Nigel J. Saunders and Sarah Butcher Severe Combined Immunodeficient (SCID) Mice in Vaccine Assessment Michael J. Dennis Clinical Trials Paddy Farrington and Elizabeth Miller Assuring the Quality and Safety of Vaccines: Regulatory Expectations for Licensing and Batch Release Elwyn Griffiths and Ivana Knezevic DNA Vaccination: An Update Douglas B. Lowrie From Vaccine Research to Manufacture: A Guide for the Researcher Nigel Allison and Howard S. Tranter Index

Customer Reviews

Handbook / Manual
By: Andrew P Robinson(Editor), Michael J Hudson(Editor), Martin P Cranage(Editor)
428 pages, b/w illustrations
Publisher: Humana Press
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