Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells. Volume two, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. The first volume, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. A companion CD provides electronic color versions of all illustrations in the book. Together, the two volumes illuminate for both novices and experts our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications.
Neural Stem Sphere Method: Induction of Neural Stem Cells and Neurons by Astrocyte-Derived Factors in Embryonic Stem Cells In Vitro, Takashi Nakayama and Nobuo Inoue. Generation and Characterization of Oligodendrocytes From Lineage-Selectable Embryonic Stem Cells In Vitro, Nathalie Billon, Christine Jolicoeur, and Martin Raff. Derivation and Characterization of Neural Cells From Embryonic Stem Cells Using Nestin Enhancer, Nibedita Lenka. Optimized Neuronal Differentiation of Murine Embryonic Stem Cells: Role of Cell Density, Matthew T. Lorincz. Generation of Inner Ear Cell Types From Embryonic Stem Cells, Marcelo N. Rivolta, Huawei Li, and Stefan Heller. Derivation of Epidermal Colony-Forming Progenitors From Embryonic Stem Cell Cultures, Tammy-Claire Troy and Kursad Turksen. Directing Epidermal Fate Selection by a Novel Co-Culture System, Tammy-Claire Troy and Kursad Turksen. In Vitro Generation of T Lymphocytes From Embryonic Stem Cells, Renee F. de Pooter, Thomas M. Schmitt, and Juan Carlos Zuniga-Pflucker. The Role of Hex in Hemangioblast and Hematopoietic Development, Rebecca J. Chan, Robert Hromas, and Mervin C. Yoder. Generation of Osteoblasts and Chondrocytes From Embryonic Stem Cells, Jitsutaro Kawaguchi. Analysis of Embryonic Stem Cell-Derived Osteogenic Cultures, Nicole L. Woll and Sarah K. Bronson. Generation of Chondrocytes From Embryonic Stem Cells, Jaspal Singh Khillan. Derivation and Characterization of Chondrocytes From Embryonic Stem Cells In Vitro, Jan Kramer, Gunnar Hargus, and Jurgen Rohwedel. Generation and Characterization of Cardiomyocytes Under Serum-Free Conditions, Cornelia Gissel, Michael Xavier Doss, Rita Hippler-Altenburg, Jurgen Hescheler, and Agapios Sachinidis. Analysis of Arrhythmic Potential of Embryonic Stem Cell-Derived Cardiomyocytes, Lijuan L. Shang, Samuel C. Dudley, Jr., and Arnold E. Pfahnl. Derivation and Characterization of Alveolar Epithelial Cells From Murine Embryonic Stem Cells In Vitro, Ali Samadikuchaksaraei and Anne E. Bishop. Derivation and Characterization of Thyrocyte-Like Cells From Embryonic Stem Cells In Vitro, Reigh-Yi Lin and Terry F. Davies. Derivation and Characterization of Gut-Like Structures From Embryonic Stem Cells, Takatsugu Yamada and Yoshiyuki Nakajima. Formation of Gut-Like Structures In Vitro From Mouse Embryonic Stem Cells, Shigeko Torihashi. In Vitro Derivation and Expansion of Endothelial Cells From Embryonic Stem Cells, Kara E. McCloskey, Steven L. Stice, and Robert M. Nerem. Differentiation of Mouse Embryonic Stem Cells Into Endothelial Cells: Genetic Selection and Potential Use In Vivo, Clotilde Gimond, Sandrine Marchetti, and Gilles Pages. Integrins and Vascular Development in Differentiated Embryonic Stem Cells In Vitro, Sheila E. Francis. TGF-b Signaling in Embryonic Stem Cell-Derived Endothelial Cells, Tetsuro Watabe, Jun K. Yamashita, Koichi Mishima, and Kohei Miyazono. The Role of the Adapter Protein SHB in Embryonic Stem Cell Differentiation Into the Pancreatic b-cell and Endothelial Lineages, Johan Saldeen, Nina A...gren, Bjorn A...kerblom, Lingge Lu, Bashir Adem, Lukasz Scdek, and Vitezslav Kriz. In Vitro Differentiation of Embryonic Stem Cells Into the Pancreatic Lineage, Przemyslaw Blyszczuk and Anna M. Wobus. Derivation and Characterization of Hepatocytes From Embryonic Stem Cells In Vitro, Shigeaki Ishizaka, Yukiteru Ouji, Masahide Yoshikawa, and Kazuki Nakatani. Differentiation of Embryonic Stem Cells to Retinal Cells In Vitro, Xing Zhao, Jianuo Liu, and Iqbal Ahmad. Derivation and Characterization of Lentoid Bodies and Retinal Pigment Epithelial Cells From Monkey Embryonic Stem Cells In Vitro, Masayo Takahashi and Masatoshi Haruta. Differentiation of Rhesus Monkey Embryonic Stem Cells in Three-Dimensional Collagen Matrix, Silvia Sihui Chen, Roberto P. Revoltella, Joshua Zimmerberg, and Leonid Margolis. Index.
From Reviews of the First Edition... "...elegantly introduces tremendous methods and protocols in ES studies...one of the most useful books that I have ever read in this field..." -Cell Biology International "...highly valuable for any scientist who wants to make a start in the exciting field but also for experienced ES cell researchers who want to widen their repertoire" -Diabetologia "...a very informative resource for any developmental or cell biologist with an interest in developments and prospects of ES cell research" -Molecular Biotechnology "...a useful companion volume to other more specialized ES cell books..." -Nature Cell Biology