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Expert researchers from academia, industry, and leading genome centers provide a comprehensive generic set of protocols for gene hunting and genome analysis. Drawing on emerging technologies in the fields of bioinformatics and proteomics, these protocols cover not only those traditionally recognized as genomics, but also early therapeutic approaches exploiting the potential of gene therapy. The highlights include methods for the analysis of differential gene expression, SNP detection, comparative genomic hybridization, and the functional analysis of genes, as well as the use of bioinformatics for gene identification and the prediction of protein function.
Construction of Microsatellite-Based, High-Resolution Genetic Maps in the Mouse, Paul A. Lyons. Genetic Analysis of Complex Traits, Stephen P. Bryant and Mathias N. Chiano. Sequence-Based Detection of Single Nucleotide Polymorphisms, Deborah A. Nickerson, Natali Kolker, Scott L. Taylor, and Mark J. Rieder. Genomic Mismatch Scanning for the Mapping of Genetic Traits, Farideh Mirzayans and Michael A. Walter. Detection of Chromosomal Abnormalities by Comparative Genomic Hybridization, Mario A. J. A. Hermsen, Marjan M. Weiss, Gerrit A. Meijer, and Jan P. A. Baak. Construction of a Bacterial Artificial Chromosome Library, Sangdun Choi and Ung-Jin Kim. Contiguation of Bacterial Clones, Sean J. Humphray, Susan J. Knaggs, and Ioannis Ragoussis. Mapping of Genomic Clones by Fluorescence In Situ Hybridization, Margaret A. Leversha. Map Integration: From a Genetic Map to a Physical Gene Map and Ultimately to the Sequence Map, Panagiotis Deloukas. Construction of Full-Length-Enriched cDNA Libraries: The Oligo-Capping Method, Yutaka Suzuki and Sumio Sugano. Construction of Transcript Maps by Somatic Cell/Radiation Hybrid Mapping: The Human Gene Map, Panagiotis Deloukas. Preparation and Screening of High-Density cDNA Arrays with Genomic Clones, Gunther Zehetner, Maria Pack, and Katja Schafer. Direct Selection of cDNAs by Genomic Clones, Daniela Toniolo. Exon Trapping: Application of a Large-Insert Multiple-Exon-Trapping System, Martin C. Wapenaar and Johan T. Den Dunnen. Sequencing Bacterial Artificial Chromosomes, David E. Harris and Lee Murphy. Finding Genes in Genomic Nucleotide Sequences by Using Bioinformatics, Yvonne J. K. Edwards and Simon M. Brocklehurst. Gene Identification Using the Pufferfish, Fugu rubripes, by Sequence Scanning, Greg Elgar. Isolation of Differentially Expressed Genes Through Subtractive Suppression Hybridization, Oliver Dorian von Stein. Isolation of Differentially Expressed Genes by Representational Difference Analysis, Christine Wallrapp and Thomas M. Gress. Expression Profiling and the Isolation of Differentially Expressed Genes by Indexing-Based Differential Display, Michael P. Starkey. Expression Profiling by Systematic High-Throughput In Situ Hybridization to Whole-Mount Embryos, Nicolas Pollet and Christof Niehrs. Expression Monitoring Using cDNA Microarrays: A General Protocol, Xing Jian Lou, Mark Schena, Frank T. Horrigan, Richard M. Lawn, and Ronald W. Davis. Prediction of Protein Structure and Function by Using Bioinformatics, Yvonne J. K. Edwards and Amanda Cottage. Identification of Novel Genes by Gene Trap Mutagenesis, Anne K. Voss and Tim Thomas. Determination of Gene Function by Homologous Recombination Using Embryonic Stem Cells and Knockout Mice, Ahmed Mansouri. Genomic Analysis Utilizing the Yeast Two-Hybrid System, Ilya G. Serebriiskii, Garabet G. Toby, Russell L. FInley, Jr., and Erica A. Golemis. Methods for Adeno-Associated Virus-Mediated Gene Transfer into Muscle, Terry J. Amiss and Richard Jude Samulski. Retroviral-Mediated Gene Transduction, Donald S. Anson. Gene Therapy Approaches to Sensitization of Human Prostate Carcinoma to Cisplatin by Adenoviral Expression of p53 and by Antisense Jun Kinase Oligonucleotide Methods, Ruth Gjerset, Ali Haghighi, Svetlana Lebedeva, and Dan Mercola. Ribozyme Gene Therapy, Leonidas A. Phylactou. Index.
The two editors of this volume have invited contributors from a variety of authors to write a laboratory manual dealing exclusively with the application of genomic methods in eukaryotes. Each chapter is presented in a standardized fashion, beginning with an introduction followed by a list of all the materials needed (including the machines and software) a detailed experimental protocol, notes (troubleshooting), and a list of references. it will be of value for those requiring step-by-step cookbook procedures for some of the methodologies described, and this definitive and authorative volume belongs in the library of every quality research institution where genomics is performed. - CHEMBIOCHEM