All Shops

Go to British Wildlife

6 issues per year 84 pages per issue Subscription only

British Wildlife is the leading natural history magazine in the UK, providing essential reading for both enthusiast and professional naturalists and wildlife conservationists. Published six times a year, British Wildlife bridges the gap between popular writing and scientific literature through a combination of long-form articles, regular columns and reports, book reviews and letters.

Subscriptions from £25 per year

Conservation Land Management

4 issues per year 44 pages per issue Subscription only

Conservation Land Management (CLM) is a quarterly magazine that is widely regarded as essential reading for all who are involved in land management for nature conservation, across the British Isles. CLM includes long-form articles, events listings, publication reviews, new product information and updates, reports of conferences and letters.

Subscriptions from £18 per year
Academic & Professional Books  Organismal to Molecular Biology  Biochemistry & Molecular Biology

RNA Isolation and Characterization Protocols

Handbook / Manual
Edited By: Ralph Rapley and David L Manning
264 pages, Illus, figs, tabs
Publisher: Humana Press
RNA Isolation and Characterization Protocols
Click to have a closer look
Select version
  • RNA Isolation and Characterization Protocols ISBN: 9780896033931 Spiralbound Dec 1998 Usually dispatched within 1-2 weeks
    £89.50
    #86825
  • RNA Isolation and Characterization Protocols ISBN: 9780896034945 Hardback Dec 1998 Usually dispatched within 1-2 weeks
    £130.50
    #73957
Selected version: £89.50
About this book Contents Customer reviews Related titles

About this book

Innovative biological investigators offer a powerful and highly useful collection of up-to-date methods for the isolation of RNA from a variety of sources, including bacterial, plant, and mammalian cells. These fine-tuned protocols take both the experienced and inexperienced investigator through the nuances of RNA manipulation, from extraction to in vitro translation. The book also includes protocols for the analysis and characterization of isolated RNA species and extensive troubleshooting hints and tips to ensure easy reproducibility. RNA Isolation and Characterization Protocols is the single best collection of easily reproducible, step-by-step RNA techniques now available for today's bench scientists.

Contents

Introduction to Isolating RNA D. E. Macfarlane and C. E. Dahle. Large and Small Scale RNA Preparations from Eukaryotic Cells W. Uckert W. Walther and U. Stein. An Improved Rapid Method of Isolating RNA from Cultured Cells D. B. Batt G. G. Carmichael and Z. Liu. Isolating RNA with the Cationic Surfactant Catrimox-14 C. E. Dahle and D. E. Macfarlane. RNA Extraction from Formalin-Fixed and Paraffin-Embedded Tissues G. Stanta S. Bonin and R. Perin. Extraction and Purification of RNA from Plant Tissues Enriched in Polysaccharides S.H. Cheng and J. R. Seemann. Isolation of Plant Mitochondrial RNA from Green Leaves F. Ye W. O. Abel and R. Reski. Extraction of RNA from Fresh and Frozen Blood B. D. M. Theophilus. Isolation of Total RNA from Bacteria J. Heptinstall. Isolation of Total RNA from Tissues or Cell Lines: Visualization in Gel T. Mukhopadhyay and J. A. Roth. Isolation of Messenger RNA S. Bryant and D. L. Manning. UV Spectrometric Analysis of Ribonucleic Acids R. Rapley and J. Heptinstall. Formaldehyde Gel Electrophoresis of Total RNA R. D. Dennis G. Lochnit and R. Geyer. Preparation of RNA Dot-Blots R. Hodge. Nonradioactive Northern Blotting R. Low. The Use of RNA Probes for the Analysis of Gene Expression: Northern Blot Hybridization and Ribonuclease Protection Assay D. Belin. Analysis of RNA by Northern Blotting Using Riboprobes R. A. K. Srivastava. RNA Quantitative Analysis from Fixed and Paraffin-Embedded Tissues G. Stanta S. Bonin and R. Utrera. Quantitative Analysis of RNA Species by PCR and Solid-Phase Minisequencing A. Suomalainen and A.-C. Syvanen. Preparation of Tissue Sections and Slides for mRNA Hybridization G. Terenghi. Detecting mRNA in Tissue Sections with Digoxigenin-Labeled Probes G. Terenghi. One-Tube RT-PCR with Sequence Specific Primes U. Pfeffer. Identification of Differentially Exposed Genes by Nonradioactive Differential Display Messenger RNA T. C. G. Bosch and J. U. Lohmann. Characterization of RNA Using Continuous RT-PCR Coupled with ELOSA F. Mallet G. Oriol and B. Mandrand. Gene Expression Analysis by CD-RT-PCR E. de Kant. Primer Extension Analysis of mRNA M. Walmsley M. Leonard and R. Patient. S1 Mapping Using Single-Stranded DNA Probes S. Viville and R. Mantovani. Measurements of Rate of Transcription in Isolated Nuclie by Nuclear Run-Off Assay R. A. K. Srivastava and G. Schonfeld. Transcription In Vitro Using Bacteriophage RNA Polymerases E. T. Schenhborn. In Vitro Translation of Messenger RNA in a Rabbit Reticulocyte Lysate Cell-Free System L. Olliver and C. D. Boyd. In Vitro Translation of Messenger RNA in a Wheat Germ Extract Cell-Free System L. Olliver A. G.-Rabie and C. D. Boyd. The Xenopus Egg Extract Translation System G. M. Matthews and A. Colman. Purification and Chracterization of Viral dsRNA Genome Profiles by Crosshybridization L.-A. Martin and P. P. C. Mertens. Index.

Customer Reviews

Handbook / Manual
Edited By: Ralph Rapley and David L Manning
264 pages, Illus, figs, tabs
Publisher: Humana Press
Media reviews
This book is aimed at clinical specialists as well as experimentalists...The references are recent. I strongly recommend its use by those involved in breast cancer treatment. It is one of the few books that deal with the management of benign breast conditions. - Oncology
Current promotions
Best of Winter 2018Handbook of the Bees of the British Isles (2-Volume Set)Order your free copy of our 2018 equipment catalogueBritish Wildlife